ArticleGenome research2004
A versatile assay for high-throughput gene expression profiling on universal array matrices.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00868192 (Phase II Trial of Pemetrexed and Bevacizumab for Recurrent Ovarian and Primary Peritoneal Carcinoma), which is not on this map. Cited by 94 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Phase II Trial of Pemetrexed and Bevacizumab for Recurrent Ovarian and Primary Peritoneal Carcinoma
Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it, 184 citations in OpenAlex.
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- Phase II study of aflibercept in recurrent malignant glioma: a North American Brain Tumor Consortium study.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2011Trial
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- Elucidating transcriptomic profiles from single-cell RNA sequencing data using nature-inspired compressed sensing.Briefings in bioinformatics · 2021Article
- Increased HLA-DR expression and cortical demyelination in MS links with HLA-DR15.Neurology(R) neuroimmunology & neuroinflammation · 2020Article
- Correlation-adjusted regression survival scores for high-dimensional variable selection.Statistics in medicine · 2019Article
- Vigorous Physical Activity Is Associated with Lower Risk of Metastatic-Lethal Progression in Prostate Cancer and Hypomethylation in theCancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2019Article
- Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies.BMC medical research methodology · 2018Article
- Integrative analysis of copy number and gene expression in breast cancer using formalin-fixed paraffin-embedded core biopsy tissue: a feasibility study.BMC genomics · 2017Article
- mRNA expression profiles of colorectal liver metastases as a novel biomarker for early recurrence after partial hepatectomy.Molecular oncology · 2016Article
- Reliable gene expression profiling of formalin-fixed paraffin-embedded breast cancer tissue (FFPE) using cDNA-mediated annealing, extension, selection, and ligation whole-genome (DASL WG) assay.BMC medical genomics · 2016Article
- Prostate tumor DNA methylation is associated with cigarette smoking and adverse prostate cancer outcomes.Cancer · 2016Article
- Expression profiles of 151 pediatric low-grade gliomas reveal molecular differences associated with location and histological subtype.Neuro-oncology · 2015Article
- Article
- Prognostic implication of morphology, cyclinE2 and proliferation in EBV-associated T/NK lymphoproliferative disease in non-immunocompromised hosts.Orphanet journal of rare diseases · 2014Article
- cFLIP expression is altered in severe corticosteroid-resistant asthma.Genomics data · 2014Article
- Transcriptome profiling of archived sectioned formalin-fixed paraffin-embedded (AS-FFPE) tissue for disease classification.PloS one · 2014Article
- CXCR4 pathway associated with family history of melanoma.Cancer causes & control : CCC · 2014Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 2 institutions in 1 country.
Funding
Abstract
We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.