ArticlePlant physiology2004
Analysis of nitric oxide signaling functions in tobacco cells challenged by the elicitor cryptogein.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 299 citations in OpenAlex.
- Nitric Oxide and Photosynthesis Interplay in Plant Interactions with Pathogens.International journal of molecular sciences · 2025Review
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- Signalling cascades choreographing petal cell death: implications for postharvest quality.Plant molecular biology · 2024Review
- Different reactions of wheat, maize, and rice plants to putrescine treatment.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
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- Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation duringPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Flagellin C decreases the expression of theFrontiers in plant science · 2022Article
- Crosstalk between abscisic acid and nitric oxide under heat stress: exploring new vantage points.Plant cell reports · 2021Review
- Review
- Membrane voltage as a dynamic platform for spatiotemporal signaling, physiological, and developmental regulation.Plant physiology · 2021Article
- Plant Nitric Oxide Signaling under Drought Stress.Plants (Basel, Switzerland) · 2021Review
- Stress signalling dynamics of the mitochondrial electron transport chain and oxidative phosphorylation system in higher plants.Annals of botany · 2020Review
- Recognition of Elicitors in Grapevine: From MAMP and DAMP Perception to Induced Resistance.Frontiers in plant science · 2019Review
- Pharmacological Strategies for Manipulating Plant CaInternational journal of molecular sciences · 2018Review
- Article
- How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels.PloS one · 2018Article
- Biochemical basis of improvement of defense in tomato plant againstPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Genetic dissection of the maize (Zea mays L.) MAMP response.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- NO and HEnvironmental science and pollution research international · 2017Article
- Moving nitrogen to the centre of plant defence against pathogens.Annals of botany · 2017Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) has recently emerged as an important cellular mediator in plant defense responses. However, elucidation of the biochemical mechanisms by which NO participates in this signaling pathway is still in its infancy. We previously demonstrated that cryptogein, an elicitor of tobacco defense responses, triggers a NO burst within minutes in epidermal sections from tobacco leaves (Nicotiana tabacum cv Xanthi). Here, we investigate the signaling events that mediate NO production, and analyze NO signaling activities in the cryptogein transduction pathway. Using flow cytometry and spectrofluorometry, we observed that cryptogein-induced NO production in tobacco cell suspensions is sensitive to nitric oxide synthase inhibitors and may be catalyzed by variant P, a recently identified pathogen-inducible plant nitric oxide synthase. NO synthesis is tightly regulated by a signaling cascade involving Ca2+ influx and phosphorylation events. Using tobacco cells constitutively expressing the Ca2+ reporter apoaequorin in the cytosol, we have shown that NO participates in the cryptogein-mediated elevation of cytosolic free Ca2+ through the mobilization of Ca2+ from intracellular stores. The NO donor diethylamine NONOate promoted an increase in cytosolic free Ca2+ concentration, which was sensitive to intracellular Ca2+ channel inhibitors. Moreover, NO appears to be involved in the pathway(s) leading to the accumulation of transcripts encoding the heat shock protein TLHS-1, the ethylene-forming enzyme cEFE-26, and cell death. In contrast, NO does not act upstream of the elicitor-induced activation of mitogen-activated protein kinase, the opening of anion channels, nor expression of GST, LOX-1, PAL, and PR-3 genes. Collectively, our data indicate that NO is intimately involved in the signal transduction processes leading to cryptogein-induced defense responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.