ArticlePlant physiology2004
Production of coumaroylserotonin and feruloylserotonin in transgenic rice expressing pepper hydroxycinnamoyl-coenzyme A:serotonin N-(hydroxycinnamoyl)transferase.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Engineered Production of Hydroxycinnamoyl Tyramine Conjugates Limits the Growth of the Pathogen Pseudomonas syringae in Arabidopsis.Plant, cell & environment · 2026Article
- Induced accumulation of serotonin in gibberellin APlant biotechnology (Tokyo, Japan) · 2025Article
- Review
- Metabolomic Evaluation of Tissue-Specific Defense Responses in Tomato Plants Modulated by PGPR-Priming againstPlants (Basel, Switzerland) · 2021Article
- Expression of the Tyrosine Hydroxylase Gene from Rat Leads to Oxidative Stress in Potato Plants.Antioxidants (Basel, Switzerland) · 2020Article
- Metabolomic Profiling of the Host Response of Tomato (International journal of molecular sciences · 2019Article
- Article
- Synthesis of avenanthramides using engineered Escherichia coli.Microbial cell factories · 2018Article
- Engineering of Escherichia coli for the synthesis of N-hydroxycinnamoyl tryptamine and serotonin.Journal of industrial microbiology & biotechnology · 2017Article
- Evolutionarily Distinct BAHD N-Acyltransferases Are Responsible for Natural Variation of Aromatic Amine Conjugates in Rice.The Plant cell · 2016Article
- Salicylic Acid Is Involved in the Basal Resistance of Tomato Plants to Citrus Exocortis Viroid and Tomato Spotted Wilt Virus.PloS one · 2016Article
- Bacterial synthesis of N-hydroxycinnamoyl phenethylamines and tyramines.Microbial cell factories · 2015Article
- Novel roles for the polyphenol oxidase enzyme in secondary metabolism and the regulation of cell death in walnut.Plant physiology · 2014Article
- Deep transcriptome sequencing of wild halophyte rice, Porteresia coarctata, provides novel insights into the salinity and submergence tolerance factors.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.).Planta · 2011Article
- Methanol elicits the biosynthesis of 4-coumaroylserotonin and feruloylserotonin in rice seedlings.Plant signaling & behavior · 2011Article
- Article
- Senescence-induced serotonin biosynthesis and its role in delaying senescence in rice leaves.Plant physiology · 2009Article
- Endosperm-specific expression of serotonin N-hydroxycinnamoyltransferase in rice.Plant foods for human nutrition (Dordrecht, Netherlands) · 2008Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic rice (Oryza sativa) plants were engineered to express a N-(hydroxycinnamoyl)transferase from pepper (Capsicum annuum), which has been shown to have hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase activity, a key enzyme in the synthesis of hydroxycinnamic acid amides, under the control of constitutive maize (Zea mays) ubiquitin promoter. The transgenic rice plants require foliar application of amines to support synthesis of hydroxycinnamic acid amides, suggestive of limiting amine substrates in rice shoots. In addition, when T2 homozygous transgenic rice plants were grown in the presence of amines or phenolic acids, two novel compounds were exclusively identified in the leaves of the transgenic plants. These compounds eluted earlier than p-coumaroyltyramine and feruloyltyramine during HPLC chromatography and were identified as p-coumaroylserotonin and feruloylserotonin by liquid chromatography/mass spectrometry and other methods. To test whether the unpredicted production of serotonin derivatives is associated with the pepper N-(hydroxycinnamoyl)transferase, the substrate specificity of the pepper enzyme was analyzed again. Purified recombinant pepper N-(hydroxycinnamoyl)transferase exhibited a serotonin N-hydroxycinnamoyltransferase (SHT) activity, synthesized p-coumaroylserotonin and feruloylserotonin in vitro, and demonstrated a low K(m) for serotonin. SHT activity was inhibited by 10 to 50 mm tyramine. In addition, SHT activity was predominantly found in the root tissues of wild-type rice in parallel with the synthesis of serotonin derivatives, suggesting that serotonin derivatives are synthesized in the root of rice. This is the first report of SHT activity and the first demonstration, to our knowledge, that serotonin derivatives can be overproduced in vivo in transgenic rice plants that express serotonin N-(hydroxycinnamoyl)transferase.
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