ArticleNucleic acids research2004
Characterization of the 3' exonuclease subunit DP1 of Methanococcus jannaschii replicative DNA polymerase D.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular basis for proofreading by the unique exonuclease domain of Family-D DNA polymerases.Nature communications · 2023Article
- Review
- Structure of the DP1-DP2 PolD complex bound with DNA and its implications for the evolutionary history of DNA and RNA polymerases.PLoS biology · 2019Article
- Elucidating functions of DP1 and DP2 subunits from the Thermococcus kodakarensis family D DNA polymerase.Extremophiles : life under extreme conditions · 2019Article
- Novel Abundant Oceanic Viruses of Uncultured Marine Group II Euryarchaeota.Current biology : CB · 2017Article
- Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography.Nature communications · 2016Article
- Pre-steady-state Kinetic Analysis of a Family D DNA Polymerase from Thermococcus sp. 9°N Reveals Mechanisms for Archaeal Genomic Replication and Maintenance.The Journal of biological chemistry · 2015Article
- Characterization of family D DNA polymerase from Thermococcus sp. 9°N.Extremophiles : life under extreme conditions · 2014Article
- Evolution of replicative DNA polymerases in archaea and their contributions to the eukaryotic replication machinery.Frontiers in microbiology · 2014Review
- Diversity of the DNA replication system in the Archaea domain.Archaea (Vancouver, B.C.) · 2014Review
- RecJ-like protein from Pyrococcus furiosus has 3'-5' exonuclease activity on RNA: implications for proofreading of 3'-mismatched RNA primers in DNA replication.Nucleic acids research · 2013Article
- Fidelity in archaeal information processing.Archaea (Vancouver, B.C.) · 2010Review
- Article
- Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol epsilon and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors.Biology direct · 2009Article
- The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins.Nucleic acids research · 2008Article
- A highly conserved family of inactivated archaeal B family DNA polymerases.Biology direct · 2008Article
- DNA replication in the archaea.Microbiology and molecular biology reviews : MMBR · 2006Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The B-subunits associated with the replicative DNA polymerases are conserved from Archaea to humans, whereas the corresponding catalytic subunits are not related. The latter belong to the B and D DNA polymerase families in eukaryotes and archaea, respectively. Sequence analysis places the B-subunits within the calcineurin-like phosphoesterase superfamily. Since residues implicated in metal binding and catalysis are well conserved in archaeal family D DNA polymerases, it has been hypothesized that the B-subunit could be responsible for the 3'-5' proofreading exonuclease activity of these enzymes. To test this hypothesis we expressed Methanococcus jannaschii DP1 (MjaDP1), the B-subunit of DNA polymerase D, in Escherichia coli, and demonstrate that MjaDP1 functions alone as a moderately active, thermostable, Mn2+-dependent 3'-5' exonuclease. The putative polymerase subunit DP2 is not required. The nuclease activity is strongly reduced by single amino acid mutations in the phosphoesterase domain indicating the requirement of this domain for the activity. MjaDP1 acts as a unidirectional, non-processive exonuclease preferring mispaired nucleotides and single-stranded DNA, suggesting that MjaDP1 functions as the proofreading exonuclease of archaeal family D DNA polymerase.
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