ArticleMolecular and cellular biology2004
Reduced fertility in male mice deficient in the zinc metallopeptidase NL1.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Neprilysin 4 controls acrosome structure and male fertility in Drosophila melanogaster.Communications biology · 2025Article
- Neprilysin 2 catalyses the degradation of natriuretic peptides despite sacubitrilat Inhibition.Scientific reports · 2025Article
- Neuropeptide inactivation regulates egg-laying behavior to influence reproductive health in Caenorhabditis elegans.Current biology : CB · 2024Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Regulation of Male Fertility by the Renin-Angiotensin System.International journal of molecular sciences · 2020Review
- Tachykinins and Kisspeptins in the Regulation of Human Male Fertility.Journal of clinical medicine · 2019Review
- Neprilysin Inhibitors and Bradykinin.Frontiers in medicine · 2018Review
- Long-term neprilysin inhibition - implications for ARNIs.Nature reviews. Cardiology · 2017Review
- Molecular characterization of firefly nuptial gifts: a multi-omics approach sheds light on postcopulatory sexual selection.Scientific reports · 2016Article
- Impact of glycosylation on the unimpaired functions of the sperm.Clinical and experimental reproductive medicine · 2015Review
- Neprilysins: an evolutionarily conserved family of metalloproteases that play important roles in reproduction in Drosophila.Genetics · 2014Article
- Amyloid-beta and Alzheimer's disease: the role of neprilysin-2 in amyloid-beta clearance.Frontiers in aging neuroscience · 2014Review
- Evolutionary rate covariation identifies new members of a protein network required for Drosophila melanogaster female post-mating responses.PLoS genetics · 2014Article
- A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia.Nature genetics · 2011Article
- Intranasal phosphoramidon increases beta-amyloid levels in wild-type and NEP/NEP2-deficient mice.Journal of molecular neuroscience : MN · 2011Article
- Neprilysin-2 is an important β-amyloid degrading enzyme.The American journal of pathology · 2011Article
- Autocrine regulation of human sperm motility by tachykinins.Reproductive biology and endocrinology : RB&E · 2010Article
- Drosophila melanogaster NEP2 is a new soluble member of the neprilysin family of endopeptidases with implications for reproduction and renal function.The Biochemical journal · 2005Article
- Strong association ofAsian journal of andrologyArticle
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the M13 family of zinc metalloendopeptidases have been shown to play critical roles in the metabolism of various neuropeptides and peptide hormones, and they have been identified as important therapeutic targets. Recently, a mouse NL1 protein, a novel member of the family, was identified and shown to be expressed mainly in the testis as a secreted protein. To define its physiological role(s), we used a gene targeting strategy to disrupt the endogenous murine Nl1 gene by homologous recombination and generate Nl1 mutant mice. The Nl1(-/-) mice were viable and developed normally, suggesting that zygotic expression of Nl1 is not required for development. However, Nl1(-/-) males produced smaller litters than their wild-type siblings, indicating specific male fertility problems. Reduced fertility may be explained by two impaired processes, decreased egg fertilization and perturbed early development of fertilized eggs. These two phenotypes did not result from gross anatomical modifications of the testis or from impaired spermatogenesis. Basic sperm parameters were also normal. Thus, our findings suggest that one of the roles of NL1 in mice is related to sperm function and that NL1 modulates the processes of fertilization and early embryonic development in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.