ArticleMolecular and cellular biology2004
The splicing factor U2AF small subunit is functionally conserved between fission yeast and humans.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effect of RNA splicing machinery gene mutations on prognosis of patients with MDS: A meta-analysis.Medicine · 2019Pooled it
- Review
- U2af1 is a haplo-essential gene required for hematopoietic cancer cell survival in mice.The Journal of clinical investigation · 2021Article
- RNA-binding proteins of COSMIC importance in cancer.The Journal of clinical investigation · 2021Review
- A Broad Overview of Signaling inCancers · 2021Review
- The zinc finger domains in U2AF26 and U2AF35 have diverse functionalities including a role in controlling translation.RNA biology · 2020Article
- The Molecular Genetics of Myeloproliferative Neoplasms.Cold Spring Harbor perspectives in medicine · 2020Review
- Functional significance of U2AF1 S34F mutations in lung adenocarcinomas.Nature communications · 2019Article
- The cancer-associated U2AF35 470A>G (Q157R) mutation creates an in-frame alternative 5' splice site that impacts splicing regulation in Q157R patients.RNA (New York, N.Y.) · 2017Article
- The power of fission: yeast as a tool for understanding complex splicing.Current genetics · 2017Review
- Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.Nucleic acids research · 2017Article
- Article
- Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation.PLoS genetics · 2016Article
- A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.Genes & development · 2015Article
- Article
- Genomic functions of U2AF in constitutive and regulated splicing.RNA biology · 2015Article
- U2AF1 mutations alter splice site recognition in hematological malignancies.Genome research · 2015Article
- New insights into the RNA-based mechanism of action of the anticancer drug 5'-fluorouracil in eukaryotic cells.PloS one · 2013Article
- A dominant role for meiosis-specific 3' RNA processing in controlling expression of a fission yeast cyclin gene.RNA (New York, N.Y.) · 2012Article
- Recurrent mutations in the U2AF1 splicing factor in myelodysplastic syndromes.Nature genetics · 2011Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The small subunit of U2AF, which functions in 3' splice site recognition, is more highly conserved than its heterodimeric partner yet is less thoroughly investigated. Remarkably, we find that the small subunit of Schizosaccharomyces pombe U2AF (U2AF(SM)) can be replaced in vivo by its human counterpart, demonstrating that the conservation extends to function. Precursor mRNAs accumulate in S. pombe following U2AF(SM) depletion in a time frame consistent with a role in splicing. A comprehensive mutational analysis reveals that all three conserved domains are required for viability. Notably, however, a tryptophan in the pseudo-RNA recognition motif implicated in a key contact with the large subunit by crystallographic data is dispensable whereas amino acids implicated in RNA recognition are critical. Mutagenesis of the two zinc-binding domains demonstrates that they are neither equivalent nor redundant. Finally, two- and three-hybrid analyses indicate that mutations with effects on large-subunit interactions are rare whereas virtually all alleles tested diminished RNA binding by the heterodimer. In addition to demonstrating extraordinary conservation of U2AF small-subunit function, these results provide new insights into the roles of individual domains and residues.
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