ArticleMolecular and cellular biology2004
Normal embryonic and germ cell development in mice lacking alpha 1,3-fucosyltransferase IX (Fut9) which show disappearance of stage-specific embryonic antigen 1.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Intervening Non-Small-Cell Lung Cancer Progression by Cell Membrane Coated Platycodin D via Regulating Hsa-miR-1246/FUT9/GSK3β Pathway.International journal of nanomedicine · 2025Article
- A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition.Scientific reports · 2021Article
- B3GNT5 is a novel marker correlated with stem-like phenotype and poor clinical outcome in human gliomas.CNS neuroscience & therapeutics · 2020Article
- Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naïve-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells.International journal of molecular sciences · 2019Article
- OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates.Stem cell reports · 2019Article
- Embryoglycan: a highly branched poly-N-acetyllactosamine in pluripotent stem cells and early embryonic cells.Glycoconjugate journal · 2017Review
- What Have We Learned from Glycosyltransferase Knockouts in Mice?Journal of molecular biology · 2016Review
- A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways.Scientific reports · 2016Article
- Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish.Developmental biology · 2014Article
- Mice lacking α1,3-fucosyltransferase 9 exhibit modulation of in vivo immune responses against pathogens.Pathology international · 2014Article
- An experimental approach to the generation of human embryonic stem cells equivalents.Molecular biotechnology · 2014Review
- Different effects of androgen on the expression of Fut1, Fut2, Fut4 and Fut9 in male mouse reproductive tract.International journal of molecular sciences · 2013Article
- The Lewis X-related α1,3-fucosyltransferase, Fut10, is required for the maintenance of stem cell populations.The Journal of biological chemistry · 2013Article
- Article
- Scaling down the size and increasing the throughput of glycosyltransferase assays: activity changes on stem cell differentiation.Analytical biochemistry · 2012Article
- Human deficiencies of fucosylation and sialylation affecting selectin ligands.Seminars in immunopathology · 2012Review
- Cancer vaccines and carbohydrate epitopes.Vaccine · 2011Review
- Stem cell glycolipids.Neurochemical research · 2011Review
- Regulation of glycosyltransferases and Lewis antigens expression by IL-1β and IL-6 in human gastric cancer cells.Glycoconjugate journal · 2011Article
- Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo.Glycoconjugate journal · 2009Article
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stage-specific embryonic antigen 1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during the 8-cell to blastocyst stages in mouse embryos and in primordial germ cells, undifferentiated embryonic stem cells, and embryonic carcinoma cells. For many years, SSEA-1 has been implicated in the development of mouse embryos as a functional carbohydrate epitope in cell-to-cell interaction during morula compaction. In a previous study, alpha 1,3-fucosyltransferase IX (Fut9) exhibited very strong activity for the synthesis of Lewis x compared to other alpha 1,3-fucosyltransferases in an in vitro substrate specificity assay. Fut4 and Fut9 transcripts were expressed in mouse embryos. The Fut9 transcript was detected in embryonic-day-13.5 gonads containing primordial germ cells, but the Fut4 transcript was not. In order to identify the role of SSEA-1 and determine the key enzyme for SSEA-1 synthesis in vivo, we have generated Fut9-deficient (Fut9(-/-)) mice. Fut9(-/-) mice develop normally, with no gross phenotypic abnormalities, and are fertile. Immunohistochemical analysis revealed an absence of SSEA-1 expression in early embryos and primordial germ cells of Fut9(-/-) mice. Therefore, we conclude that expression of the SSEA-1 epitope in the developing mouse embryo is not essential for embryogenesis in vivo.
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