ArticleMolecular and cellular biology2004
Polypyrimidine tract binding protein modulates efficiency of polyadenylation.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 108 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
108 citing papers in PubMed, 164 citations in OpenAlex.
- A conserved 3'UTR short motif regulates gene expression in vertebrates.Nucleic acids research · 2026Article
- Local Protein Synthesis at Synapses: A Driver for Synapse Diversification.Journal of neurochemistry · 2025Review
- Fateful Decisions of Where to Cut the Line: Pathology Associated with Aberrant 3' End Processing and Transcription Termination.Journal of molecular biology · 2025Review
- Regulation of alternative splicing and polyadenylation in neurons.Life science alliance · 2023Review
- Reviewing PTBP1 Domain Modularity in the Pre-Genomic Era: A Foundation to Guide the Next Generation of Exploring PTBP1 Structure-Function Relationships.International journal of molecular sciences · 2023Review
- Article
- Transcriptome sequencing suggests that pre-mRNA splicing counteracts widespread intronic cleavage and polyadenylation.NAR genomics and bioinformatics · 2023Article
- Review
- Polypyrimidine tract binding protein 1 regulates the activation of mouse CD8 T cells.European journal of immunology · 2022Article
- The landscape of alternative polyadenylation in single cells of the developing mouse embryo.Nature communications · 2021Article
- RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3.The Journal of reproduction and development · 2020Article
- Activation and inhibition of nonsense-mediated mRNA decay control the abundance of alternative polyadenylation products.Nucleic acids research · 2020Article
- A transient α-helix in the N-terminal RNA recognition motif of polypyrimidine tract binding protein senses RNA secondary structure.Nucleic acids research · 2020Article
- In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol.Infectious agents and cancer · 2020Article
- mRNA Processing: An Emerging Frontier in the Regulation of Pancreatic β Cell Function.Frontiers in genetics · 2020Review
- Cell Cycle Kinase Polo Is Controlled by a Widespread 3' Untranslated Region Regulatory Sequence in Drosophila melanogaster.Molecular and cellular biology · 2019Article
- Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.The Journal of clinical investigation · 2019Article
- RNA-binding protein Ptbp1 is essential for BCR-mediated antibody production.International immunology · 2019Article
- Alternative polyadenylation dependent function of splicing factor SRSF3 contributes to cellular senescence.Aging · 2019Article
- PTBP3 contributes to the metastasis of gastric cancer by mediating CAV1 alternative splicing.Cell death & disease · 2018Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polypyrimidine tract binding protein (PTB) is a major hnRNP protein with multiple roles in mRNA metabolism, including regulation of alternative splicing and internal ribosome entry site-driven translation. We show here that a fourfold overexpression of PTB results in a 75% reduction of mRNA levels produced from transfected gene constructs with different polyadenylation signals (pA signals). This effect is due to the reduced efficiency of mRNA 3' end cleavage, and in vitro analysis reveals that PTB competes with CstF for recognition of the pA signal's pyrimidine-rich downstream sequence element. This may be analogous to its role in alternative splicing, where PTB competes with U2AF for binding to pyrimidine-rich intronic sequences. The pA signal of the C2 complement gene unusually possesses a PTB-dependent upstream sequence, so that knockdown of PTB expression by RNA interference reduces C2 mRNA expression even though PTB overexpression still inhibits polyadenylation. Consequently, we show that PTB can act as a regulator of mRNA expression through both its negative and positive effects on mRNA 3' end processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.