ArticleMolecular and cellular biology2004
Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- Due-B Is dispensable for early development and genome duplication in vertebrates.bioRxiv : the preprint server for biology · 2025Article
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Order from clutter: selective interactions at mammalian replication origins.Nature reviews. Genetics · 2017Review
- DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila.Nucleic acids research · 2015Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- G4 motifs affect origin positioning and efficiency in two vertebrate replicators.The EMBO journal · 2014Article
- Activation of a human chromosomal replication origin by protein tethering.Nucleic acids research · 2013Article
- Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- Evaluating genome-scale approaches to eukaryotic DNA replication.Nature reviews. Genetics · 2010Review
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Intrinsically bent DNA sites in the Drosophila melanogaster third chromosome amplified domain.Molecular genetics and genomics : MGG · 2009Article
- DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.Molecular genetics and genomics : MGG · 2007Article
- An origin of DNA replication in the promoter region of the human fragile X mental retardation (FMR1) gene.Molecular and cellular biology · 2007Article
- Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites.Experimental cell research · 2007Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- Differential binding of replication proteins across the human c-myc replicator.Molecular and cellular biology · 2006Article
- DNA bending in the replication zone of the C3 DNA puff amplicon of Rhynchosciara americana (Diptera: Sciaridae).Molecular biology reports · 2006Article
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A small DNA fragment containing the high-frequency initiation region (IR) ori-beta from the hamster dihydrofolate reductase locus functions as an independent replicator in ectopic locations in both hamster and human cells. Conversely, a fragment of the human lamin B2 locus containing the previously mapped IR serves as an independent replicator at ectopic chromosomal sites in hamster cells. At least four defined sequence elements are specifically required for full activity of ectopic ori-beta in hamster cells. These include an AT-rich element, a 4-bp sequence located within the mapped IR, a region of intrinsically bent DNA located between these two elements, and a RIP60 protein binding site adjacent to the bent region. The ori-beta AT-rich element is critical for initiation activity in human, as well as hamster, cells and can be functionally substituted for by an AT-rich region from the human lamin B2 IR that differs in nucleotide sequence and length. Taken together, the results demonstrate that two mammalian replicators can be activated at ectopic sites in chromosomes of another mammal and lead us to speculate that they may share functionally related elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.