ArticleMolecular and cellular biology2004
Key role of Ser562/661 in Snf1-dependent regulation of Cat8p in Saccharomyces cerevisiae and Kluyveromyces lactis.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- TheAging · 2023Article
- Sucrose-nonfermenting 1 kinase activates histone acetylase GCN5 to promote cellulase production in Trichoderma.Applied microbiology and biotechnology · 2023Article
- The polyHIS Tract of Yeast AMPK Coordinates Carbon Metabolism with Iron Availability.International journal of molecular sciences · 2023Article
- Article
- The multiple effects of REG1 deletion and SNF1 overexpression improved the production of S-adenosyl-L-methionine in Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors.Frontiers in microbiology · 2022Article
- Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.Current genetics · 2021Article
- Spontaneous mutations inMicrobial cell (Graz, Austria) · 2018Article
- An essential domain of an early-diverged RNA polymerase II functions to accurately decode a primitive chromatin landscape.Nucleic acids research · 2017Article
- Caloric Restriction Extends Yeast Chronological Life Span by Optimizing the Snf1 (AMPK) Signaling Pathway.Molecular and cellular biology · 2017Article
- Network reconstruction and validation of the Snf1/AMPK pathway in baker's yeast based on a comprehensive literature review.NPJ systems biology and applications · 2015Article
- Divergent Evolution of the Transcriptional Network Controlled by Snf1-Interacting Protein Sip4 in Budding Yeasts.PloS one · 2015Article
- Triggering respirofermentative metabolism in the crabtree-negative yeast Pichia guilliermondii by disrupting the CAT8 gene.Applied and environmental microbiology · 2014Article
- Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis.Microbial cell factories · 2013Article
- Snf1/AMPK regulates Gcn5 occupancy, H3 acetylation and chromatin remodelling at S. cerevisiae ADY2 promoter.Biochimica et biophysica acta · 2012Article
- Transcriptional regulation of nonfermentable carbon utilization in budding yeast.FEMS yeast research · 2010Review
- Glucose signaling-mediated coordination of cell growth and cell cycle in Saccharomyces cerevisiae.Sensors (Basel, Switzerland) · 2010Review
- Differential roles of the glycogen-binding domains of beta subunits in regulation of the Snf1 kinase complex.Eukaryotic cell · 2010Article
- Article
- Effects of ADH2 overexpression in Saccharomyces bayanus during alcoholic fermentation.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Utilization of nonfermentable carbon sources by Kluyveromyces lactis and Saccharomyces cerevisiae requires the Snf1p kinase and the Cat8p transcriptional activator, which binds to carbon source-responsive elements of target genes. We demonstrate that KlSnf1p and KlCat8p from K. lactis interact in a two-hybrid system and that the interaction is stronger with a kinase-dead mutant form of KlSnf1p. Of two putative phosphorylation sites in the KlCat8p sequence, serine 661 was identified as a key residue governing KlCat8p regulation. Serine 661 is located in the middle homology region, a regulatory domain conserved among zinc cluster transcription factors, and is part of an Snf1p consensus phosphorylation site. Single mutations at this site are sufficient to completely change the carbon source regulation of the KlCat8p transactivation activity observed. A serine-to-glutamate mutant form mimicking constitutive phosphorylation results in a nearly constitutively active form of KlCat8p, while a serine-to-alanine mutation has the reverse effect. Furthermore, it is shown that KlCat8p phosphorylation depends on KlSNF1. The Snf1-Cat8 connection is evolutionarily conserved: mutation of corresponding serine 562 of ScCat8p gave similar results in S. cerevisiae. The enhanced capacity of ScCat8S562E to suppress the phenotype caused by snf1 strengthens the hypothesis of direct phosphorylation of Cat8p by Snf1p. Unlike that of S. cerevisiae ScCAT8, KlCAT8 transcription is not carbon source regulated, illustrating the prominent role of posttranscriptional regulation of Cat8p in K. lactis.
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