ArticleProceedings of the National Academy of Sciences of the United States of America2004
Axonal growth of embryonic stem cell-derived motoneurons in vitro and in motoneuron-injured adult rats.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 1 of them a synthesis that pooled it.
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Who cites it
82 citing papers in PubMed, 1 synthesis or guideline pooled it, 234 citations in OpenAlex.
- Neural Stem/Progenitor Cell Therapy in Patients and Animals with Amyotrophic Lateral Sclerosis: A Systematic Review and Meta-analysis.Molecular neurobiology · 2025Pooled it
- Human motor neurons generated from neural stem cells delay clinical onset and prolong life in ALS mouse model.PloS one · 2014Trial
- Intraparenchymal stem cell transplantation for spinal cord injury: a review of clinical trials.Regenerative medicine · 2026Review
- Unraveling the Potential of Stem Cell Therapy in Motor Neuron Disease: A Narrative Review.CNS & neurological disorders drug targets · 2026Review
- Neural stem cells in adult neurogenesis and their therapeutic applications in neurodegenerative disorders: a concise review.Frontiers in molecular medicine · 2025Review
- Disease Mechanisms and Therapeutic Approaches in SMARD1-Insights from Animal Models and Cell Models.Biomedicines · 2024Review
- Regenerative medicine improve neurodegenerative diseases.Cell and tissue banking · 2023Review
- Motor neuron replacement therapy for amyotrophic lateral sclerosis.Neural regeneration research · 2022Review
- Potential of Cellular Therapy for ALS: Current Strategies and Future Prospects.Frontiers in cell and developmental biology · 2022Review
- Gene Therapy Approach with an Emphasis on Growth Factors: Theoretical and Clinical Outcomes in Neurodegenerative Diseases.Molecular neurobiology · 2022Review
- Fully Characterized Mature Human iPS- and NMP-Derived Motor Neurons Thrive Without Neuroprotection in the Spinal Contusion Cavity.Frontiers in cellular neuroscience · 2021Article
- A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction.Journal of clinical medicine · 2020Article
- Crosstalk between stem cell and spinal cord injury: pathophysiology and treatment strategies.Stem cell research & therapy · 2019Review
- Simplified in vitro engineering of neuromuscular junctions between rat embryonic motoneurons and immortalized human skeletal muscle cells.Stem cells and cloning : advances and applications · 2019Article
- New perspectives on the mechanisms establishing the dorsal-ventral axis of the spinal cord.Current topics in developmental biology · 2019Review
- Advances in Patient-Specific Induced Pluripotent Stem Cells Shed Light on Drug Discovery for Amyotrophic Lateral Sclerosis.Cell transplantation · 2018Review
- Deriving Dorsal Spinal Sensory Interneurons from Human Pluripotent Stem Cells.Stem cell reports · 2018Article
- Transplantation of Human Embryonic Stem Cells in Patients with Multiple Sclerosis and Lyme Disease.The American journal of case reports · 2016Article
- Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units.Science advances · 2016Article
- Human Embryonic Stem Cell Therapy in Chronic Spinal Cord Injury: A Retrospective Study.Clinical and translational science · 2016Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We generated spinal motoneurons from embryonic stem (ES) cells to determine the developmental potential of these cells in vitro and their capacity to replace motoneurons in the adult mammalian spinal cord. ES cell-derived motoneurons extended long axons, formed neuromuscular junctions, and induced muscle contraction when cocultured with myoblasts. We transplanted motoneuron-committed ES cells into the spinal cords of adult rats with motoneuron injury and found that approximately 3,000 ES cell-derived motoneurons (25% of input) survived for >1 month in the spinal cord of each animal. ES cell-derived axonal growth was inhibited by myelin, and this inhibition was overcome by administration of dibutyryl cAMP (dbcAMP) or a Rho kinase inhibitor in vitro and in vivo. In transplanted rats infused with dbcAMP, approximately 80 ES cell-derived motor axons were observed within the ventral roots of each animal, whereas none were observed in transplanted rats not treated with dbcAMP. Because these cells replicate many of the developmental and mature features of true motoneurons, they are an important biological tool to understand formation of motor units in vitro and a potential therapeutic tool to reconstitute neural circuits in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.