ArticleThe Journal of cell biology2004
Fascin-mediated propulsion of Listeria monocytogenes independent of frequent nucleation by the Arp2/3 complex.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 87 citations in OpenAlex.
- Brush border intermicrovillar adhesion limits bacteria attachment to the small intestine brush border.bioRxiv : the preprint server for biology · 2025Article
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.bioRxiv : the preprint server for biology · 2023Article
- Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.Communications biology · 2021Article
- Fascin-induced actin protrusions are suppressed by dendritic networks in giant unilamellar vesicles.Molecular biology of the cell · 2021Article
- CD2AP links actin to PI3 kinase activity to extend epithelial cell height and constrain cell area.The Journal of cell biology · 2020Article
- From solution to surface to filament: actin flux into branched networks.Biophysical reviews · 2018Review
- Article
- In vitro investigations on extracellular proteins secreted by Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome.Acta veterinaria Scandinavica · 2017Article
- CRMP-1 enhances EVL-mediated actin elongation to build lamellipodia and the actin cortex.The Journal of cell biology · 2017Article
- Quantitative Analysis of Filament Branch Orientation in Listeria Actin Comet Tails.Biophysical journal · 2016Article
- Collapsin Response Mediator Protein-1 Regulates Arp2/3-dependent Actin Assembly.The Journal of biological chemistry · 2016Article
- Bacterial spread from cell to cell: beyond actin-based motility.Trends in microbiology · 2015Review
- Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Fascin confers resistance to Listeria infection in dendritic cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Cyclase-associated protein (CAP) acts directly on F-actin to accelerate cofilin-mediated actin severing across the range of physiological pH.The Journal of biological chemistry · 2012Article
- α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction.The Journal of cell biology · 2012Article
- Actin filament attachments for sustained motility in vitro are maintained by filament bundling.PloS one · 2012Article
- Optimal orientation in branched cytoskeletal networks.Journal of mathematical biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Actin-dependent propulsion of Listeria monocytogenes is thought to require frequent nucleation of actin polymerization by the Arp2/3 complex. We demonstrate that L. monocytogenes motility can be separated into an Arp2/3-dependent nucleation phase and an Arp2/3-independent elongation phase. Elongation-based propulsion requires a unique set of biochemical factors in addition to those required for Arp2/3-dependent motility. We isolated fascin from brain extracts as the only soluble factor required in addition to actin during the elongation phase for this type of movement. The nucleation reaction assembles a comet tail of branched actin filaments directly behind the bacterium. The elongation-based reaction generates a hollow cylinder of parallel bundles that attach along the sides of the bacterium. Bacteria move faster in the elongation reaction than in the presence of Arp2/3, and the rate is limited by the concentration of G-actin. The biochemical and structural differences between the two motility reactions imply that each operates through distinct biochemical and biophysical mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.