ArticleThe Journal of cell biology2004
Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 124 citations in OpenAlex.
- Transcriptional and post-transcriptional control of odorant receptor choice in ants.Current biology : CB · 2023Article
- Onepot-Seq: capturing single-cell transcriptomes simultaneously in a continuous medium via transient localization of mRNA.Nucleic acids research · 2022Article
- Nuclear mechanisms of gene expression control: pre-mRNA splicing as a life or death decision.Current opinion in genetics & development · 2021Review
- Splicing at the phase-separated nuclear speckle interface: a model.Nucleic acids research · 2021Review
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- Uncoupling of nucleo-cytoplasmic RNA export and localization during stress.Nucleic acids research · 2019Article
- Multiple covalent fluorescence labeling of eukaryotic mRNA at the poly(A) tail enhances translation and can be performed in living cells.Nucleic acids research · 2019Article
- Nuclear speckles: molecular organization, biological function and role in disease.Nucleic acids research · 2017Review
- Nucleic Acid-Based Nanodevices in Biological Imaging.Annual review of biochemistry · 2016Review
- RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.Current protocols in chemical biology · 2016Article
- ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.Nucleic acids research · 2015Article
- Control of mammalian gene expression by selective mRNA export.Nature reviews. Molecular cell biology · 2015Review
- Quantum Chemical Calculations and Experimental Validation of the Photoclick Reaction for Fluorescent Labeling of the 5' cap of Eukaryotic mRNAs.ChemistryOpen · 2015Article
- Live-cell imaging of mammalian RNAs with Spinach2.Methods in enzymology · 2015Article
- An immunocytochemical study of interchromatin granule clusters in early mouse embryos.BioMed research international · 2013Article
- To the pore and through the pore: a story of mRNA export kinetics.Biochimica et biophysica acta · 2012Review
- Article
- Probes for intracellular RNA imaging in live cells.Methods in enzymology · 2012Article
- Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2'-O-methyl RNA probes in living cells.Nucleic acids research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many of the protein factors that play a role in nuclear export of mRNAs have been identified, but still little is known about how mRNAs are transported through the cell nucleus and which nuclear compartments are involved in mRNA transport. Using fluorescent 2'O-methyl oligoribonucleotide probes, we investigated the mobility of poly(A)+ RNA in the nucleoplasm and in nuclear speckles of U2OS cells. Quantitative analysis of diffusion using photobleaching techniques revealed that the majority of poly(A)+ RNA move throughout the nucleus, including in and out of speckles (also called SC-35 domains), which are enriched for splicing factors. Interestingly, in the presence of the transcription inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, the association of poly(A)+ RNA with speckles remained dynamic. Our results show that RNA movement is energy dependent and that the proportion of nuclear poly(A)+ RNA that resides in speckles is a dynamic population that transiently interacts with speckles independent of the transcriptional status of the cell. Rather than the poly(A)+ RNA within speckles serving a stable structural role, our findings support the suggestion of a more active role of these regions in nuclear RNA metabolism and/or transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.