ArticleJournal of virology2004
Copatching and lipid raft association of different viral glycoproteins expressed on the surfaces of pseudorabies virus-infected cells.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Proteomic Comparison of Three Wild-Type Pseudorabies Virus Strains and the Attenuated Bartha Strain Reveals Reduced Incorporation of Several Tegument Proteins in Bartha Virions.Journal of virology · 2022Article
- Structural Domains of the Herpes Simplex Type 1 gD Protein that Restrict HIV-1 Particle Infectivity.Journal of virology · 2021Article
- UL11 Protein Is a Key Participant of the Duck Plague Virus in Its Life Cycle.Frontiers in microbiology · 2021Article
- Expression of the Pseudorabies Virus gB Glycoprotein Triggers NK Cell Cytotoxicity and Increases Binding of the Activating NK Cell Receptor PILRβ.Journal of virology · 2019Article
- With or without rafts? Alternative views on cell membranes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Mono-ubiquitylated ORF45 Mediates Association of KSHV Particles with Internal Lipid Rafts for Viral Assembly and Egress.PLoS pathogens · 2015Article
- Pseudorabies virus triggers glycoprotein gE-mediated ERK1/2 activation and ERK1/2-dependent migratory behavior in T cells.Journal of virology · 2015Article
- Role of Us9 phosphorylation in axonal sorting and anterograde transport of pseudorabies virus.PloS one · 2013Article
- Antibodies to cell surface proteins redirect intracellular trafficking pathways.Experimental and molecular pathology · 2011Article
- Porcine sialoadhesin (CD169/Siglec-1) is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages.PloS one · 2011Article
- Cholesterol dependence of pseudorabies herpesvirus entry.Current microbiology · 2011Article
- Optimal replication of human cytomegalovirus correlates with endocytosis of glycoprotein gpUL132.Journal of virology · 2010Article
- Relationships between plasma membrane microdomains and HIV-1 assembly.Biology of the cell · 2010Review
- y Human herpesvirus 6 envelope components enriched in lipid rafts: evidence for virion-associated lipid rafts.Virology journal · 2009Article
- Comparison of the pseudorabies virus Us9 protein with homologs from other veterinary and human alphaherpesviruses.Journal of virology · 2009Article
- Targeting of pseudorabies virus structural proteins to axons requires association of the viral Us9 protein with lipid rafts.PLoS pathogens · 2008Article
- Article
- Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line.Virology journal · 2007Article
- Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins.Journal of virology · 2006Article
- Vaccinia virus penetration requires cholesterol and results in specific viral envelope proteins associated with lipid rafts.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudorabies virus (PRV) is a swine alphaherpesvirus that is closely related to human herpes simplex virus (HSV). Both PRV and HSV express a variety of viral envelope glycoproteins in the plasma membranes of infected cells. Here we show that at least four major PRV glycoproteins (gB, gC, gD, and gE) in the plasma membrane of infected swine kidney cells and monocytes seem to be linked, since monospecific antibody-induced patching of any one of these proteins results in copatching of the others. Further, for all four PRV glycoproteins, monospecific antibody-induced patches were enriched in GM1, a typical marker of lipid raft microdomains, but were excluded for transferrin receptor, a nonraft marker, suggesting that these viral proteins may associate with lipid rafts. However, only gB and, to a lesser extent, gE were found in lipid raft fractions by using detergent floatation assays, indicating that gC and gD do not show strong lipid raft association. Addition of methyl-beta-cyclodextrin (MCD), a cholesterol-depleting agent that is commonly used to disrupt lipid rafts, only slightly reduced copatching efficiency between the different viral proteins, indicating that other factors, perhaps tegument-glycoprotein interactions, may be important for the observed copatching events. On the other hand, MCD strongly reduced polarization of the antibody-induced viral glycoprotein patches to a cap structure, a gE-dependent process that has been described for specific PRV- and HSV-infected cells. Therefore, we hypothesize that efficient gE-mediated capping of antibody-antigen patches may require the lipid raft-associated signal transduction machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.