ArticleJournal of virology2004
Rapid and sensitive detection of retrovirus entry by using a novel luciferase-based content-mixing assay.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.Biochemistry research international · 2024Review
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- Development of an enzyme-linked immunosorbent spot assay to measure serum-neutralizing antibodies against coxsackievirus B3.Clinical and vaccine immunology : CVI · 2014Article
- Article
- Ebolavirus requires acid sphingomyelinase activity and plasma membrane sphingomyelin for infection.Journal of virology · 2012Article
- GRB2 interaction with the ecotropic murine leukemia virus receptor, mCAT-1, controls virus entry and is stimulated by virus binding.Journal of virology · 2012Article
- Host-encoded reporters for the detection and purification of multiple enveloped viruses.Journal of virological methods · 2010Article
- Exosomes packaging APOBEC3G confer human immunodeficiency virus resistance to recipient cells.Journal of virology · 2009Article
- Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm.Retrovirology · 2008Review
- Bioluminescent virion shells: new tools for quantitation of AAV vector dynamics in cells and live animals.Gene therapy · 2008Article
- Phosphoinositide-3 kinase-Akt pathway controls cellular entry of Ebola virus.PLoS pathogens · 2008Article
- Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling.Virology · 2008Article
- Determining functionally important amino acid residues of the E1 protein of Venezuelan equine encephalitis virus.Journal of molecular modeling · 2006Article
- The perforin-dependent immunological synapse allows T-cell activation-dependent tumor targeting by MLV vector particles.Gene therapy · 2006Article
- Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane.Molecular biology of the cell · 2005Article
- Liposome reconstitution of a minimal protein-mediated membrane fusion machine.The EMBO journal · 2005Article
- Actin- and myosin-driven movement of viruses along filopodia precedes their entry into cells.The Journal of cell biology · 2005Article
- Efficient functional pseudotyping of oncoretroviral and lentiviral vectors by Venezuelan equine encephalitis virus envelope proteins.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a novel assay that permits measurement of entry of murine leukemia virus and pseudotypes with greater sensitivity and more rapidly than previously possible. To achieve this, we encapsulated a sensitive reporter enzyme, luciferase, directly into fully infectious, intact viral particles. The enzyme is specifically targeted to the viral lumen, as a C-terminal fusion on the viral envelope protein. Only when the incorporated luciferase is released from the viral lumen and gains access to its substrates is light emitted and readily detected. When cells are perfused with luciferin, quantitative measurements of entry can be made in real time on live cells. Uniquely, the amount of cell-bound virus can be determined in the same assay by addition of detergent to expose the luciferase. We demonstrate that virus carrying a mutation in the fusion peptide binds normally to cells but is unable to infect them and gives no entry signal. Using this assay, we show that inhibitors of endosomal acidification inhibit signal from vesicular stomatitis virus pseudotypes but not murine leukemia virus, consistent with a pH-independent mode of entry for the latter virus. Additionally, the fusion kinetics are rapid, with a half-life of 25 min after a delay of 10 to 15 min. The future use of this assay will permit a detailed examination of the entry mechanism of viruses and provide a convenient platform to discover novel entry inhibitors. The design also permits packaging of potential therapeutic protein cargoes into functional virus particles and their specific delivery to cellular targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.