ArticleJournal of clinical pathology2004
In vivo and in vitro studies on Anaplasma phagocytophilum infection of the myeloid cells of a patient with chronic myelogenous leukaemia and human granulocytic ehrlichiosis.
Article in Journal of clinical pathology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Human granulocytotropic anaplasmosis-A systematic review and analysis of the literature.PLoS neglected tropical diseases · 2024Pooled it
- An in-house nucleic acid test for detection of Anaplasma phagocytophilum in whole blood donor samples.Transfusion · 2026Article
- Culture and other direct detection methods to diagnose human granulocytic anaplasmosis.American journal of clinical pathology · 2025Article
- Human Granulocytic Anaplasmosis-A Systematic Review of Published Cases.Microorganisms · 2022Review
- A rare case of delayed anaplasma phagocytophilum-induced pancytopenia: A diagnostic conundrum.Annals of medicine and surgery (2012) · 2022Article
- Negative impact of the COVID-19 pandemic on the timely diagnosis of tick-borne infections.Diagnostic microbiology and infectious disease · 2021Article
- First reported case of Ehrlichia ewingii involving human bone marrow.Journal of clinical microbiology · 2014Article
- Anaplasma phagocytophilum--a widespread multi-host pathogen with highly adaptive strategies.Frontiers in cellular and infection microbiology · 2013Review
- Anaplasma phagocytophilum infection induces apoptosis in HL-60 cells.World journal of microbiology & biotechnology · 2011Article
- Mechanisms of obligatory intracellular infection with Anaplasma phagocytophilum.Clinical microbiology reviews · 2011Review
- Anaplasma phagocytophilum AptA modulates Erk1/2 signalling.Cellular microbiology · 2011Article
- Neutrophils exposed to A. phagocytophilum under shear stress fail to fully activate, polarize, and transmigrate across inflamed endothelium.American journal of physiology. Cell physiology · 2010Article
- Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells.Infection and immunity · 2009Article
- Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis.BMC genomics · 2008Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe occurrence of human granulocytic ehrlichiosis (HGE) in a patient with chronic myelogenous leukaemia (CML) provided an opportunity to study whether Anaplasma phagocytophilum, the aetiological agent of HGE, infects mature or immature cells, both in vivo and in vitro.
methodsDiagnosis of HGE was confirmed by culture, polymerase chain reaction (PCR), detection of intragranulocytic inclusions, and serology. The infection rates of different myelogenous stages of granulocytic differentiation were determined by microscopy. Anaplasma phagocytophilum infection of the bone marrow was analysed by PCR, culture, and microscopy. In addition, the in vitro growth of A phagocytophilum in the patient's granulocytes and in HL-60 cells (a promyelocytic leukaemia cell line) was compared.
resultsPretreatment blood smears showed that mature granulocytic cells had a higher infection rate with A phagocytophilum than did immature cells. In the original inoculation of the patient's cells into HL-60 cells to isolate A phagocytophilum, the bacterium grew faster in the patient's leukaemic cells than in HL-60 cells. Anaplasma phagocytophilum inclusions were rarely seen in bone marrow granulocytes and PCR was negative. In vitro, two A phagocytophilum isolates grew faster in the patient's granulocytes than in HL-60 cells.
conclusionsThe superior growth in CML cells compared with HL-60 cells suggests that A phagocytophilum preferentially infects mature granulocytes. The higher infection rate of the patient's mature versus immature granulocytes before treatment and the minimal level of infection of the patient's bone marrow support this. It is possible that the primary site of infection in HGE is the peripheral mature granulocytic population.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.