ArticleWorld journal of gastroenterology2004
Association of low p16INK4a and p15INK4b mRNAs expression with their CpG islands methylation with human hepatocellular carcinogenesis.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 41 citations in OpenAlex.
- Pooled it
- P16 gene hypermethylation and hepatocellular carcinoma: a systematic review and meta-analysis.World journal of gastroenterology · 2011Pooled it
- INK4 cyclin-dependent kinase inhibitors as potential prognostic biomarkers and therapeutic targets in hepatocellular carcinoma.Bioscience reports · 2022Article
- Review
- Investigation of the Effect of 5-Aza-2'-Deoxycytidine in Comparison to and in Combination with Trichostatin A onInternational journal of preventive medicine · 2021Article
- Indole Derivative Interacts with Estrogen Receptor Beta and Inhibits Human Ovarian Cancer Cell Growth.Molecules (Basel, Switzerland) · 2020Article
- Modified CDKN2B (p15) and CDKN2A (p16) DNA methylation profiles in urban pesticide applicators.Environmental science and pollution research international · 2019Article
- The innovative regularity and role of p16 methylation in blood during HCC development.Journal of Cancer · 2018Article
- Quantitative assessment of aberrantCancer management and research · 2018Review
- Article
- Molecular alterations in the carcinogenesis and progression of hepatocellular carcinoma: Tumor factors and background liver factors.Oncology letters · 2016Article
- P15 gene methylation in hepatocellular carcinomas: a systematic review and meta-analysis.International journal of clinical and experimental medicine · 2015Review
- DNA methylation: potential biomarker in Hepatocellular Carcinoma.Biomarker research · 2014Article
- Comparison of methylation profiling in cancerous and their corresponding normal tissues from korean patients with breast cancer.Annals of laboratory medicine · 2013Article
- Analysis of the methylation patterns of the p16 INK4A, p15 INK4B, and APC genes in gastric adenocarcinoma patients from a Brazilian population.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2013Article
- HBV induced HCC: major risk factors from genetic to molecular level.BioMed research international · 2013Review
- Assessment of the effect of betaine on p16 and c-myc DNA methylation and mRNA expression in a chemical induced rat liver cancer model.BMC cancer · 2009Article
- Progression of hypermethylation of the p16(INK4A) gene from normal liver to nontumorous liver and hepatocellular carcinoma: an evaluation using quantitative PCR analysis.Digestive diseases and sciences · 2009Article
- Tumor suppressor and hepatocellular carcinoma.World journal of gastroenterology · 2008Review
- Molecular Pathogenesis of Hepatitis-B-virus-associated Hepatocellular Carcinoma.Gut and liver · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the significance of p16 and p15 transcription suppression with hypermethylation of their genes' 5'CpG islands during human hepatocellular carcinogenesis.
methodsThe mRNA expression levels of p16 and p15 genes were evaluated in cancerous, para-cancerous and non-cancerous tissues of 20 HCC, 3 normal liver tissues from 3 accidentally died healthy adults using semi-quantitatively Northern blot. The methylation status was also assessed with methylation specific PCR.
resultsp16 mRNA expression level was decreased in the cancerous tissues in 60% (12/20) of HCC patients, of which 2 cases had no p16 mRNA detected, 5 cases (25%) displayed variation in the order of cancerous<para-cancerous<non-cancerous liver tissues. p15 mRNA expression level was decreased in the cancerous tissues in 50% (10/20) HCC patients, of which one case had no p15 mRNA detected, 4 cases (20%) displayed variation in the order of cancerous<para-cancerous<non-cancerous liver tissues. In cancerous, para-cancerous and non-cancerous tissues, p16 promoter CpG islands hypermethylation occurred 65%, 60% and 35%, while p15 promoter CpG islands hypermethylation occurred 50%, 40% and 25%. Of 12 HCCs with lower p16 mRNA expression level, 11 cases showed p16 promoter CpG islands methylation (91.6%). Hundred percent (10/10) HCCs with lower p15 mRNA expression level showed p15 promoter CpG islands methylation. Significant correlation between 5'CpG islands methylation and p16/p15 mRNA expression suppression was found. The decreased expression of p16/p15 mRNA or methylation of p16/p15 promoters 5'CpG island was significantly correlate with poor differentiation of HCC (P=0.0083, 0.0102, 0.00271, 0.0218, respectively, P<0.05).
conclusionp16 and p15 genes transcriptional inactivation might play an important role in hepatocarcinogenesis. 5'CpG islands methylation might be the major mechanism of p16 and p15 genes inactivation in primary HCC in the studied population. 5'CpG islands methylation of p16 and p15 genes might be an early event in hepatocarcinogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.