ArticleBiophysical journal2004
Tissue electroporation: quantification and analysis of heterogeneous transport in multicellular environments.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- Gene Electrotransfer Efficiency in 2D and 3D Cancer Cell Models Using Different Electroporation Protocols: A Comparative Study.Pharmaceutics · 2023Article
- Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells.Pharmaceutics · 2022Article
- Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination.Cancers · 2022Review
- Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens.Nucleic acids research · 2018Article
- Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.The Journal of membrane biology · 2018Article
- Development of an Acute Method to Deliver Transgenes Into the Brains of AdultFrontiers in neural circuits · 2018Article
- Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues.Journal of biomolecular screening · 2015Review
- Calcium Electroporation: Evidence for Differential Effects in Normal and Malignant Cell Lines, Evaluated in a 3D Spheroid Model.PloS one · 2015Article
- Cell-cell proximity effects in multi-cell electroporation.Biomicrofluidics · 2014Article
- 3D spheroids' sensitivity to electric field pulses depends on their size.The Journal of membrane biology · 2013Article
- Hyaluronidase and collagenase increase the transfection efficiency of gene electrotransfer in various murine tumors.Human gene therapy · 2012Article
- Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells.Biochimica et biophysica acta · 2010Article
- Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer.The Journal of membrane biology · 2010Article
- Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.Integrative biology : quantitative biosciences from nano to macro · 2010Article
- Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 2 - In vivo developments and present clinical applications.Biophysical reviews · 2009Review
- Kinetics of transmembrane transport of small molecules into electropermeabilized cells.Biophysical journal · 2008Article
- Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonication.Ultrasound in medicine & biology · 2007Article
- Efficient electroporation of DNA and protein into confluent and differentiated epithelial cells in culture.Traffic (Copenhagen, Denmark) · 2007Article
- Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus.BMC developmental biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although electroporation is gaining increased attention as a technology to enhance clinical chemotherapy and gene therapy of tissues, direct measurements of electroporation-mediated transport in multicellular environments are lacking. In this study, we used multicellular tumor spheroids of DU145 prostate cancer cells as a model tissue to measure the levels and distribution of molecular uptake in a multicellular environment as a function of electrical and other parameters. These measurements, and subsequent analysis, were used to test the hypothesis that cells in a multicellular environment respond to electroporation in a heterogeneous manner that differs from isolated cells in suspension due to differences in cell state, local solute concentration, and local electric field. In support of the hypothesis, molecular uptake was consistently lower for cells within spheroids than cells in dilute suspension and was spatially heterogeneous, with progressively less uptake observed for cells located deeper within spheroid interiors. Reduced uptake and heterogeneity can be explained quantitatively by accounting for the effects of cell size on transmembrane voltage and cell volume, limited extracellular solute reservoir, heterogeneous field strength due to influence of neighboring cells, and diffusional lag times.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.