ArticleBiophysical journal2004
Imaging the activity and localization of single voltage-gated Ca(2+) channels by total internal reflection fluorescence microscopy.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Docking and Activity of DNA Polymerase on Solid-State Nanopores.ACS sensors · 2022Article
- PunctaSpecks: A tool for automated detection, tracking, and analysis of multiple types of fluorescently labeled biomolecules.Cell calcium · 2020Article
- Noise analysis of cytosolic calcium image data.Cell calcium · 2020Article
- Applications of FLIKA, a Python-based image processing and analysis platform, for studying local events of cellular calcium signaling.Biochimica et biophysica acta. Molecular cell research · 2019Article
- Measuring Sharp Waves and Oscillatory Population Activity With the Genetically Encoded Calcium Indicator GCaMP6f.Frontiers in cellular neuroscience · 2019Article
- Neuroprotective effects of TRPA1 channels in the cerebral endothelium following ischemic stroke.eLife · 2018Article
- TraceSpecks: A Software for Automated Idealization of Noisy Patch-Clamp and Imaging Data.Biophysical journal · 2018Article
- Analyzing optical imaging of CaCell calcium · 2017Article
- Surface dynamics of voltage-gated ion channels.Channels (Austin, Tex.) · 2016Review
- Imaging the dynamics of individual electropores.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.Cell calcium · 2015Article
- Picomolar sensitivity to inositol trisphosphate in Xenopus oocytes.Cell calcium · 2015Article
- Imaging local Ca2+ signals in cultured mammalian cells.Journal of visualized experiments : JoVE · 2015Article
- Analyzing and Modeling the Kinetics of Amyloid Beta Pores Associated with Alzheimer's Disease Pathology.PloS one · 2015Article
- Article
- An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.Cell calcium · 2014Article
- Down-regulation of CaV1.2 channels during hypertension: how fewer CaV1.2 channels allow more Ca(2+) into hypertensive arterial smooth muscle.The Journal of physiology · 2013Article
- TRP channel Ca(2+) sparklets: fundamental signals underlying endothelium-dependent hyperpolarization.American journal of physiology. Cell physiology · 2013Review
- Imaging large cohorts of single ion channels and their activity.Frontiers in endocrinology · 2013Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The patch-clamp technique has enabled functional studies of single ion channels, but suffers limitations including lack of spatial information and inability to independently monitor currents from more than one channel. Here, we describe the use of total internal reflection fluorescence microscopy as an alternative, noninvasive approach to optically monitor the activity and localization of multiple Ca(2+)-permeable channels in the plasma membrane. Images of near-membrane Ca(2+) signals were obtained from >100 N-type channels expressed within restricted areas (80 x 80 micro m) of Xenopus oocytes, thereby permitting simultaneous resolution of their gating kinetics, voltage dependence, and localization. Moreover, this technique provided information inaccessible by electrophysiological means, demonstrating that N-type channels are immobile in the membrane, show a patchy distribution, and display diverse gating kinetics even among closely adjacent channels. Total internal reflection fluorescence microscopy holds great promise for single-channel recording of diverse voltage- and ligand-gated Ca(2+)-permeable channels in the membrane of neurons and other isolated or cultured cells, and has potential for high-throughput functional analysis of single channels.
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