ArticleBiophysical journal2004
Cross-bridge number, position, and angle in target zones of cryofixed isometrically active insect flight muscle.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- The 3D structure of fibrous material is fully restorable from its X-ray diffraction pattern.IUCrJ · 2021Article
- Troponin Revealed: Uncovering the Structure of the Thin Filament On-Off Switch in Striated Muscle.Biophysical journal · 2021Review
- Through thick and thin: dual regulation of insect flight muscle and cardiac muscle compared.Journal of muscle research and cell motility · 2019Review
- Synchrotron Radiation X-ray Diffraction Techniques Applied to Insect Flight Muscle.International journal of molecular sciences · 2018Review
- Modeling CaFrontiers in physiology · 2016Article
- Asymmetric myosin binding to the thin filament as revealed by a fluorescent nanocircuit.Archives of biochemistry and biophysics · 2013Article
- Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.PloS one · 2012Article
- Quasiperiodic distribution of rigor cross-bridges along a reconstituted thin filament in a skeletal myofibril.Biophysical journal · 2011Article
- Regulating the contraction of insect flight muscle.Journal of muscle research and cell motility · 2011Review
- Evidence for pre- and post-power stroke of cross-bridges of contracting skeletal myofibrils.Biophysical journal · 2011Article
- Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.PloS one · 2010Article
- Coupling of adjacent tropomyosins enhances cross-bridge-mediated cooperative activation in a markov model of the cardiac thin filament.Biophysical journal · 2010Article
- Article
- Methods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle.Journal of structural biology · 2009Article
- Force-generating cross-bridges during ramp-shaped releases: evidence for a new structural state.Biophysical journal · 2009Article
- Cardiac thin filament regulation.Pflugers Archiv : European journal of physiology · 2008Review
- Invertebrate muscles: thin and thick filament structure; molecular basis of contraction and its regulation, catch and asynchronous muscle.Progress in neurobiology · 2008Review
- Similarities and differences between frozen-hydrated, rigor acto-S1 complexes of insect flight and chicken skeletal muscles.Journal of molecular biology · 2008Article
- Reverse actin sliding triggers strong myosin binding that moves tropomyosin.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Ca-activation and stretch-activation in insect flight muscle.Biophysical journal · 2004Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Electron micrographic tomograms of isometrically active insect flight muscle, freeze substituted after rapid freezing, show binding of single myosin heads at varying angles that is largely restricted to actin target zones every 38.7 nm. To quantify the parameters that govern this pattern, we measured the number and position of attached myosin heads by tracing cross-bridges through the three-dimensional tomogram from their origins on 14.5-nm-spaced shelves along the thick filament to their thin filament attachments in the target zones. The relationship between the probability of cross-bridge formation and axial offset between the shelf and target zone center was well fitted by a Gaussian distribution. One head of each myosin whose origin is close to an actin target zone forms a cross-bridge most of the time. The probability of cross-bridge formation remains high for myosin heads originating within 8 nm axially of the target zone center and is low outside 12 nm. We infer that most target zone cross-bridges are nearly perpendicular to the filaments (60% within 11 degrees ). The results suggest that in isometric contraction, most cross-bridges maintain tension near the beginning of their working stroke at angles near perpendicular to the filament axis. Moreover, in the absence of filament sliding, cross-bridges cannot change tilt angle while attached nor reach other target zones while detached, so may cycle repeatedly on and off the same actin target monomer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.