ArticleThe Biochemical journal2004
Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
- The LiaFSR-SpxA2 regulatory axis governs bile salt resistance and virulence in Group BVirulence · 2026Article
- Antifungal Potential of Peptide-Enriched Fractions Isolated from Capsicum frutescens Seeds Against Yeasts of the Genus Candida.Probiotics and antimicrobial proteins · 2026Article
- Exploring the Healing Powers of Histatins: From Oral Health to Therapeutics.International journal of molecular sciences · 2025Review
- Mechanistic Insight into the Anti-Bacterial/Anti-Biofilm Effects of Low Chlorhexidine Concentrations onMicroorganisms · 2024Article
- Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.Metallomics : integrated biometal science · 2023Article
- Anti-Bacterial and Anti-Biofilm Activities of Anandamide against the CariogenicInternational journal of molecular sciences · 2023Article
- GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization, mitochondria-targeting and -activating properties of human salivary histatin 1.International journal of oral science · 2022Article
- Anti-Bacterial Properties of Cannabigerol TowardFrontiers in microbiology · 2021Article
- Salivary Histatin 1 and 2 Are Targeted to Mitochondria and Endoplasmic Reticulum in Human Cells.Cells · 2020Article
- Whole-Genome Approach to Understanding the Mechanism of Action of a Histatin 5-Derived Peptide.Antimicrobial agents and chemotherapy · 2020Article
- Octominin: A Novel Synthetic Anticandidal Peptide Derived from Defense Protein of Octopus minor.Marine drugs · 2020Article
- Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid.Journal of food science and technology · 2016Article
- Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp.PloS one · 2016Article
- How does it kill?: understanding the candidacidal mechanism of salivary histatin 5.Eukaryotic cell · 2014Review
- Improved bioactivity of antimicrobial peptides by addition of amino-terminal copper and nickel (ATCUN) binding motifs.ChemMedChem · 2014Article
- Properties and mechanisms of action of naturally occurring antifungal peptides.Cellular and molecular life sciences : CMLS · 2013Review
- Salivary histatin 5 internalization by translocation, but not endocytosis, is required for fungicidal activity in Candida albicans.Molecular microbiology · 2010Article
- Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth.Free radical biology & medicine · 2010Review
- In vitro reactive oxygen species production by histatins and copper(I,II).Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Hepatocyte necrosis induced by oxidative stress and IL-1 alpha release mediate carcinogen-induced compensatory proliferation and liver tumorigenesis.Cancer cell · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of action of antimicrobial peptides is still a matter of debate. The formation of ROS (reactive oxygen species) has been suggested to be the crucial step in the fungicidal mechanism of a number of antimicrobial peptides, including histatin 5 and lactoferrin-derived peptides. In the present study we have investigated the effects of histatin 5 and of a more amphipathic synthetic derivative, dhvar4, on the generation of ROS in the yeast Candida albicans, using dihydroethidium as an indicator for ROS. With both peptides, a substantial enhancement of fluorescence was observed. However, TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a cell-permeant ROS scavenger, did not have an inhibitory effect on killing or on the enhancement of fluorescence. Furthermore, antimycin and azide, which have been reported to induce ROS in vitro, were not able to enhance the dihydroethidium fluorescence, while chlorhexidine, a non-specific antiseptic agent, enhanced dihydroethidium fluorescence to the same extent as did the peptides. Fluorescence microscopy showed the fluorescence enhancement to be a consequence of the release of unbound preformed ethidium from the mitochondrial matrix within the cell. It is concluded that ROS do not play a role in the histatin 5-mediated killing of C. albicans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.