ArticleCurrent genetics2004
Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth.
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.International journal of molecular sciences · 2023Article
- Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans.Scientific reports · 2020Article
- Molecular Mechanisms of Conidial Germination inMicrobiology and molecular biology reviews : MMBR · 2020Review
- Avidity-driven polarity establishment via multivalent lipid-GTPase module interactions.The EMBO journal · 2019Article
- Patterns of Conservation and Diversification in the Fungal Polarization Network.Genome biology and evolution · 2018Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway.Molecular and cellular biology · 2015Article
- Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells.International journal of oncology · 2013Article
- Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Cdc42p and Fus2p act together late in yeast cell fusion.Molecular biology of the cell · 2012Article
- Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.Nature cell biology · 2012Article
- Cytoskeletal polarity mediates localized induction of the heart progenitor lineage.Nature cell biology · 2011Article
- Dual modes of cdc42 recycling fine-tune polarized morphogenesis.Developmental cell · 2009Article
- The Cryptococcus neoformans Rho-GDP dissociation inhibitor mediates intracellular survival and virulence.Infection and immunity · 2008Article
- The Rho GDI Rdi1 regulates Rho GTPases by distinct mechanisms.Molecular biology of the cell · 2008Article
- Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii.BMC microbiology · 2008Article
- Localization of RHO-4 indicates differential regulation of conidial versus vegetative septation in the filamentous fungus Neurospora crassa.Eukaryotic cell · 2007Article
- Use of bimolecular fluorescence complementation to study in vivo interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae.Eukaryotic cell · 2007Article
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is clustered at polarized growth sites or is extracted from membranes by Rho guanine-nucleotide dissociation inhibitors (RhoGDIs) at specific times in the cell cycle. To address these points, localization studies were performed in Saccharomyces cerevisiae using green fluorescent protein (GFP)-tagged Cdc42p and the RhoGDI Rdi1p. GFP-Rdi1p localized to polarized growth sites at specific times of the cell cycle but not to other sites of Cdc42p localization. Overexpression of Rdi1p led to loss of GFP-Cdc42p from internal and plasma membranes. This effect was mediated through the Cdc42p Rho-insert domain, which was also implicated in interactions with the Bni1p scaffold protein. These data suggested that Rdi1p functions in cell cycle-specific Cdc42p membrane detachment. Additional genetic and time-lapse microscopy analyses implicated nucleotide binding in the clustering of Cdc42p. Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.