ArticleThe Biochemical journal2004
Characterization of transgenic mice with widespread overexpression of spermine synthase.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Myeloid GPSM1 regulates atherosclerosis progression by governing monocyte and macrophage activation and chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Arginine, Agmatine, and Polyamines: Key Regulators of Conceptus Development in Mammals.Advances in experimental medicine and biology · 2021Article
- Functions of Polyamines in Mammals.The Journal of biological chemistry · 2016Review
- Spermine synthase overexpression in vivo does not increase susceptibility to DMBA/TPA skin carcinogenesis or Min-Apc intestinal tumorigenesis.Cancer biology & therapy · 2012Article
- Article
- Overexpression of ornithine decarboxylase decreases ventricular systolic function during induction of cardiac hypertrophy.Amino acids · 2012Article
- Defining the role of polyamines in colon carcinogenesis using mouse models.Journal of carcinogenesis · 2011Article
- Transgenic overexpression of LARGE induces α-dystroglycan hyperglycosylation in skeletal and cardiac muscle.PloS one · 2010Article
- Mutation in archain 1, a subunit of COPI coatomer complex, causes diluted coat color and Purkinje cell degeneration.PLoS genetics · 2010Article
- Spermine synthase.Cellular and molecular life sciences : CMLS · 2010Review
- Mammalian polyamine metabolism and function.IUBMB life · 2009Review
- Mouse models to investigate the function of spermine.Communicative & integrative biology · 2009Article
- Spermine synthase deficiency leads to deafness and a profound sensitivity to alpha-difluoromethylornithine.The Journal of biological chemistry · 2009Article
- Crystal structure of human spermine synthase: implications of substrate binding and catalytic mechanism.The Journal of biological chemistry · 2008Article
- Leishmania donovani polyamine biosynthetic enzyme overproducers as tools to investigate the mode of action of cytotoxic polyamine analogs.Antimicrobial agents and chemotherapy · 2007Article
- A time- and dose-dependent STAT1 expression system.BMC biotechnology · 2006Article
- Overproduction of cardiac S-adenosylmethionine decarboxylase in transgenic mice.The Biochemical journal · 2006Article
- Animal disease models generated by genetic engineering of polyamine metabolism.Journal of cellular and molecular medicineReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A widespread increase in SpmS (spermine synthase) activity has been produced in transgenic mice using a construct in which the human SpmS cDNA was placed under the control of a composite CMV-IE (cytomegalovirus immediate early gene) enhancer-chicken beta-actin promoter. Four separate founder CAG/SpmS mice were studied. Transgenic expression of SpmS was found in all of the tissues examined, but the relative SpmS activities varied widely according to the founder animal and the tissue studied. Very large increases in SpmS activity were seen in many tissues. SpdS (spermidine synthase) activity was not affected. Although there was a statistically significant decline in spermidine content and increase in spermine, the alterations were small compared with the increase in SpmS activity. These results provide strong support for the concept that the levels of the higher polyamines spermidine and spermine are not determined only by the relative activities of the two aminopropyltransferases. Other factors such as availability of the aminopropyl donor substrate decarboxylated S-adenosylmethionine and possibly degradation or excretion must also influence the spermidine/spermine ratio. No deleterious effects of SpmS overexpression were seen. The mice had normal growth, fertility and behaviour up to the age of 12 months. However, breeding the CAG/SpmS mice with MHC (alpha-myosin heavy chain)/AdoMetDC (S-adenosylmethionine decarboxylase) mice, which have a large increase in S-adenosylmethionine decarboxylase expression in heart, was lethal. In contrast, breeding the CAG/SpmS mice with MHC/ODC (L-ornithine decarboxylase) mice, which have a large increase in cardiac ornithine decarboxylase expression, had a protective effect in preventing the small decrease in viability of the MHC/ODC mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.