ArticleInfection and immunity2004
Genetic evidence that Legionella pneumophila RpoS modulates expression of the transmission phenotype in both the exponential phase and the stationary phase.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 87 citations in OpenAlex.
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- LD-transpeptidase-mediated cell envelope remodeling enables developmental transitions and survival inJournal of bacteriology · 2025Article
- Iron-depleting nutritional immunity controls extracellular bacterial replication in Legionella pneumophila infections.Nature communications · 2024Article
- Ecology ofBiofilm · 2024Review
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- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
- Knowledge to Predict Pathogens:Microorganisms · 2022Review
- Development of a Fluorescent Tool for StudyingMicroorganisms · 2021Article
- The Life Cycle ofFrontiers in cellular and infection microbiology · 2018Review
- The Flagellar Regulon ofFrontiers in cellular and infection microbiology · 2017Review
- The Legionella pneumophila GIG operon responds to gold and copper in planktonic and biofilm cultures.PloS one · 2017Article
- The phtC-phtD locus equips Legionella pneumophila for thymidine salvage and replication in macrophages.Infection and immunity · 2014Article
- The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.Frontiers in microbiology · 2014Review
- Metabolism of the vacuolar pathogen Legionella and implications for virulence.Frontiers in cellular and infection microbiology · 2014Article
- Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits.Infection and immunity · 2013Article
- ArgR-regulated genes are derepressed in the Legionella-containing vacuole.Journal of bacteriology · 2010Article
- Article
- The Legionella pneumophila LetA/LetS two-component system exhibits rheostat-like behavior.Infection and immunity · 2010Article
- ppGpp conjures bacterial virulence.Microbiology and molecular biology reviews : MMBR · 2010Review
- Temporal and spatial trigger of post-exponential virulence-associated regulatory cascades by Legionella pneumophila after bacterial escape into the host cell cytosol.Environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The opportunistic pathogen Legionella pneumophila alternates between two states: replication within phagocytes and transmission between host amoebae or macrophages. In broth cultures that model this life cycle, during the replication period, CsrA inhibits expression of transmission traits. When nutrients become limiting, the alarmone (p)ppGpp accumulates and the sigma factors RpoS and FliA and the positive activators LetA/S and LetE promote differentiation to the transmissible form. Here we show that when cells enter the postexponential growth phase, RpoS increases expression of the transmission genes fliA, flaA, and mip, factors L. pneumophila needs to establish a new replication niche. In contrast, in exponential (E)-phase cells whose (p)ppGpp levels are low, rpoS inhibits expression of transmission traits, on the basis of three separate observations. First, rpoS RNA levels peak in the E phase, suggestive of a role for RpoS during replication. Second, in multiple copies, rpoS decreases the amounts of csrA, letE, fliA, and flaA transcripts and inhibits the transmission traits of motility, infectivity, and cytotoxicity. Third, rpoS blocks expression of cytotoxicity and motility by E-phase bacteria that have been induced to express the LetA activator ectopically. The data are discussed in the context of a model in which the alarmone (p)ppGpp enables RpoS to outcompete other sigma factors for binding to RNA polymerase to promote transcription of transmission genes, while LetA/S acts in parallel to relieve CsrA posttranscriptional repression of the transmission regulon. By coupling transcriptional and posttranscriptional control pathways, intracellular L. pneumophila could respond to stress by rapidly differentiating to a transmissible form.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.