ArticleMolecular microbiology2004
Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation.
Article in Molecular microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Intracellular microbial shifts during COVID-19 infection and longitudinal recovery revealed by single-cell RNA sequencing.iScience · 2026Article
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Regulation of Ribosomal Protein Synthesis in Mycobacteria: The Autogenous Control ofInternational journal of molecular sciences · 2021Article
- Autogenous regulation in vivo of theRNA (New York, N.Y.) · 2020Article
- rRNA Mimicry in RNA Regulation of Gene Expression.Microbiology spectrum · 2018Review
- Article
- RNA regulators responding to ribosomal protein S15 are frequent in sequence space.Nucleic acids research · 2016Article
- Co-evolution of Bacterial Ribosomal Protein S15 with Diverse mRNA Regulatory Structures.PLoS genetics · 2015Article
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Discovery and validation of novel and distinct RNA regulators for ribosomal protein S15 in diverse bacterial phyla.BMC genomics · 2014Article
- Folding a stable RNA pseudoknot through rearrangement of two hairpin structures.Nucleic acids research · 2014Article
- Bacterial RNA motif in the 5' UTR of rpsF interacts with an S6:S18 complex.RNA (New York, N.Y.) · 2014Article
- Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation.PLoS biology · 2013Article
- Changes in translational efficiency is a dominant regulatory mechanism in the environmental response of bacteria.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Hfq affects mRNA levels independently of degradation.BMC molecular biology · 2010Article
- Processing and stability of inducibly expressed rpsO mRNA derivatives in Bacillus subtilis.Journal of bacteriology · 2009Article
- A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo.RNA (New York, N.Y.) · 2008Article
- Towards deciphering the principles underlying an mRNA recognition code.Current opinion in structural biology · 2008Review
- Ribosome biogenesis and the translation process in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2007Review
- Viral RNA pseudoknots: versatile motifs in gene expression and replication.Nature reviews. Microbiology · 2007Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The ribosomal protein S15 binds to 16S rRNA, during ribosome assembly, and to its own mRNA (rpsO mRNA), affecting autocontrol of its expression. In both cases, the RNA binding site is bipartite with a common subsite consisting of a G*U/G-C motif. The second subsite is located in a three-way junction in 16S rRNA and in the distal part of a stem forming a pseudoknot in Escherichia coli rpsO mRNA. To determine the extent of mimicry between these two RNA targets, we determined which amino acids interact with rpsO mRNA. A plasmid carrying rpsO (the S15 gene) was mutagenized and introduced into a strain lacking S15 and harbouring an rpsO-lacZ translational fusion. Analysis of deregulated mutants shows that each subsite of rpsO mRNA is recognized by a set of amino acids known to interact with 16S rRNA. In addition to the G*U/G-C motif, which is recognized by the same amino acids in both targets, the other subsite interacts with amino acids also involved in contacts with helix H22 of 16S rRNA, in the region adjacent to the three-way junction. However, specific S15-rpsO mRNA interactions can also be found, probably with A(-46) in loop L1 of the pseudoknot, demonstrating that mimicry between the two targets is limited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.