ArticleRNA (New York, N.Y.)2004
Principles of 3' splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Pairwise Engineering of Tandemly Aligned Self-Splicing Group I Introns for Analysis and Control of Their Alternative Splicing.Biomolecules · 2023Article
- Group II Introns: Flexibility and Repurposing.Frontiers in molecular biosciences · 2022Article
- The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.RNA (New York, N.Y.) · 2015Article
- Evolution of group II introns.Mobile DNA · 2015Article
- Novel RNA structural features of an alternatively splicing group II intron from Clostridium tetani.RNA (New York, N.Y.) · 2014Article
- Bacterial genome instability.Microbiology and molecular biology reviews : MMBR · 2014Review
- Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani.Nucleic acids research · 2014Article
- Crystal structure of a group II intron in the pre-catalytic state.Nature structural & molecular biology · 2012Article
- Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.Nucleic acids research · 2011Article
- A conserved 3' extension in unusual group II introns is important for efficient second-step splicing.Nucleic acids research · 2009Article
- Group II introns in eubacteria and archaea: ORF-less introns and new varieties.RNA (New York, N.Y.) · 2008Article
- Survey of group I and group II introns in 29 sequenced genomes of the Bacillus cereus group: insights into their spread and evolution.Nucleic acids research · 2008Article
- Group II intron in Bacillus cereus has an unusual 3' extension and splices 56 nucleotides downstream of the predicted site.Nucleic acids research · 2007Article
- Multiple physical forms of excised group II intron RNAs in wheat mitochondria.Nucleic acids research · 2006Article
- Self-splicing of a group IIC intron: 5' exon recognition and alternative 5' splicing events implicate the stem-loop motif of a transcriptional terminator.Nucleic acids research · 2006Article
- Unusual group II introns in bacteria of the Bacillus cereus group.Journal of bacteriology · 2005Article
- Quantitative analysis of group II intron expression and splicing in Lactococcus lactis.Applied and environmental microbiology · 2005Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the self-splicing properties of two introns from the bacterium Bacillus anthracis. One intron (B.a.I1) splices poorly in vitro despite having typical structural motifs, while the second (B.a.I2) splices well while having apparently degenerated features. The spliced exons of B.a.I2 were sequenced, and splicing was found to occur at a 3' site shifted one nucleotide from the expected position, thus restoring missing gamma-gamma' and IBS3-EBS3 pairings, but leaving the two conserved exonic ORFs out of frame. Because of the unexpected splice site, the principles for 3' intron definition were examined, which showed that the 3' splice site is flexible but contingent on gamma-gamma' and IBS3-EBS3 pairings, and can be as far away as four nucleotides from the wild-type site. Surprisingly, alternative splicing occurs at position +4 for wild-type B.a.I2 intron, both in vitro and in vivo, and the alternative event fuses the two conserved exon ORFs, presumably leading to translation of the downstream ORF. The finding suggests that the structural irregularities of B.a.I2 may be an adaptation to facilitate gene expression in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.