ArticleProtein science : a publication of the Protein Society2004
Proteins can convert to beta-sheet in single crystals.
Article in Protein science : a publication of the Protein Society, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Raman Spectroscopic Insights of Phase-Separated Insulin Aggregates.ACS physical chemistry Au · 2024Article
- Non-invasive identification of combined salinity stress and stalk rot disease caused by Colletotrichum graminicola in maize using Raman spectroscopy.Scientific reports · 2023Article
- Raman spectroscopy detects chemical differences between potato tubers produced under normal and heat stress growing conditions.Frontiers in plant science · 2023Article
- Raman Spectroscopy and Improved Inception Network for Determination of FHB-Infected Wheat Kernels.Foods (Basel, Switzerland) · 2022Article
- Raman-Based Diagnostics of Stalk Rot Disease of Maize Caused byFrontiers in plant science · 2021Article
- Non-invasive identification of potato varieties and prediction of the origin of tuber cultivation using spatially offset Raman spectroscopy.Analytical and bioanalytical chemistry · 2020Article
- Raman observation of a molecular signaling pathway of apoptotic cells induced by photothermal therapy.Chemical science · 2019Article
- Rapid, quantitative determination of aggregation and particle formation for antibody drug conjugate therapeutics with label-free Raman spectroscopy.Analytica chimica acta · 2019Article
- Article
- Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death.PloS one · 2018Article
- Envisaging the Structural Elevation in the Early Event of Oligomerization of Disordered Amyloid β Peptide.ACS omega · 2017Article
- Plasmon-enhanced Raman spectroscopic metrics forChemical science · 2017Article
- Confocal microscopy on the beamline: novel three-dimensional imaging and sample positioning.Journal of applied crystallography · 2012Article
- Kinetic crystallography by Raman microscopy.Biochimica et biophysica acta · 2011Review
- Different intermediate populations formed by tazobactam, sulbactam, and clavulanate reacting with SHV-1 beta-lactamases: Raman crystallographic evidence.Journal of the American Chemical Society · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Raman microscopy was used to follow conformational changes in single protein crystals. Crystals of native insulin and of the 5S and 12S subunits of the enzyme transcarboxylase showed a mixture of Raman marker bands signifying alpha-helix, beta-sheet and nonordered secondary structure. However, by reducing the S-S bonds in the insulin crystal, or by lowering the pH for the 5S crystal, or by soaking substrates into the 12S crystal, the secondary structure in each crystal became predominantly beta-sheet. The beta-form crystals could be dissolved only with difficulty and yielded high-molecular weight protein aggregates, indicating that the beta-sheet formation involves intermolecular contacts. Although their morphology appeared unchanged, the crystals no longer diffracted X-rays. Using crystals that had not been exposed to laser light, the dye thioflavin T formed highly fluorescent complexes with the "beta-transformed" crystals but not with the native crystals.
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