ArticleProceedings of the National Academy of Sciences of the United States of America2004
Isotopolog perturbation techniques for metabolic networks: metabolic recycling of nutritional glucose in Drosophila melanogaster.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- An isotopically-labelled temporal mass spectrometry imaging data analysis workflow to reveal glucose spatial metabolism patterns in bovine lens tissue.Scientific reports · 2024Article
- Cellular metabolic reprogramming controls sugar appetite in Drosophila.Nature metabolism · 2020Article
- Metabolomic Studies inGenetics · 2017Review
- Decoding Biosynthetic Pathways in Plants by Pulse-Chase Strategies Using (13)CO₂ as a Universal Tracer †.Metabolites · 2016Review
- Chloroplast-localized 6-phosphogluconate dehydrogenase is critical for maize endosperm starch accumulation.Journal of experimental botany · 2013Article
- Novel diagnostics of metabolic dysfunction detected in breath and plasma by selective isotope-assisted labeling.Metabolism: clinical and experimental · 2012Article
- The Drosophila estrogen-related receptor directs a metabolic switch that supports developmental growth.Cell metabolism · 2011Article
- Nectar formation and floral nectary anatomy of Anigozanthos flavidus: a combined magnetic resonance imaging and spectroscopy study.Journal of experimental botany · 2008Article
- 13C isotopologue perturbation studies of Listeria monocytogenes carbon metabolism and its modulation by the virulence regulator PrfA.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers.BMC biochemistry · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster strain Oregon-R(*) was grown on standard medium supplemented with [U-(13)C(6)]glucose. One to two days after hatching, flies were extracted with water. Glucose was isolated chromatographically from the extract and was analyzed by (13)C NMR spectroscopy. All (13)C signals of the isolated glucose were multiplets arising by (13)C(13)C coupling. Based on a comprehensive analysis of the coupling constants and heavy isotope shifts in glucose, the integrals of individual (13)C signal patterns afforded the concentrations of certain groups of (13)C isotopologs. These data were deconvoluted by a genetic algorithm affording the abundances of all single-labeled and of 15 multiply labeled isotopologs. Among the latter group, seven isotopologs were found at concentrations >0.1 mol % with [1,2-(13)C(2)]glucose as the most prominent species. The multiply (13)C-labeled glucose isotopologs are caused by metabolic remodeling of the proffered glucose via a complex network of catabolic and anabolic processes involving glycolysis and/or passage through the pentose phosphate, the Cori cycle and/or the citrate cycle. The perturbation method described can be adapted to a wide variety of experimental systems and isotope-labeled precursors.
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