ArticleNucleic acids research2004
Human telomerase catalyzes nucleolytic primer cleavage.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded Protein.bioRxiv : the preprint server for biology · 2026Article
- Oxidative guanine base damage regulates human telomerase activity.Nature structural & molecular biology · 2016Article
- The functional requirement of two structural domains within telomerase RNA emerged early in eukaryotes.Nucleic acids research · 2016Article
- Defective repair of uracil causes telomere defects in mouse hematopoietic cells.The Journal of biological chemistry · 2015Article
- Specific features of telomerase RNA from Hansenula polymorpha.RNA (New York, N.Y.) · 2013Article
- Telomere lengthening and other functions of telomerase.Acta naturae · 2012Article
- Telomerase promotes efficient cell cycle kinetics and confers growth advantage to telomerase-negative transformed human cells.Oncogene · 2012Article
- Expression of telomerase & its significance in the diagnosis of pancreatic cancer.The Indian journal of medical research · 2012Review
- Similarities between long interspersed element-1 (LINE-1) reverse transcriptase and telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- G-quadruplex formation at the 3' end of telomere DNA inhibits its extension by telomerase, polymerase and unwinding by helicase.Nucleic acids research · 2011Article
- The MRT-1 nuclease is required for DNA crosslink repair and telomerase activity in vivo in Caenorhabditis elegans.The EMBO journal · 2009Article
- Structural features of mouse telomerase RNA are responsible for the lower activity of mouse telomerase versus human telomerase.The Biochemical journal · 2006Article
- trt-1 is the Caenorhabditis elegans catalytic subunit of telomerase.PLoS genetics · 2006Article
- Regulation of 5' template usage and incorporation of noncognate nucleotides by human telomerase.RNA (New York, N.Y.) · 2005Article
- Telomerase can act as a template- and RNA-independent terminal transferase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- An anchor site-type defect in human telomerase that disrupts telomere length maintenance and cellular immortalization.Molecular biology of the cell · 2005Article
- A human-Tetrahymena pseudoknot chimeric telomerase RNA reconstitutes a nonprocessive enzyme in vitro that is defective in telomere elongation.Nucleic acids research · 2005Article
- Relaxed primer specificity associated with reverse transcriptases encoded by the pFOXC retroplasmids of Fusarium oxysporum.Eukaryotic cell · 2004Article
- Biochemical properties of Trypanosoma cruzi telomerase.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase is a reverse transcriptase that uses an integral RNA molecule to add de novo G-rich repeats onto telomeric DNA, or onto nontelomeric DNA generated during chromosome fragmentation and breakage events. A telomerase-mediated DNA substrate cleavage activity has been reported in ciliates and yeasts. Nucleolytic cleavage may serve a proofreading function, enhance processivity or ensure that nontemplate telomerase RNA sequences are not copied into DNA. We identified and characterized a human telomerase-mediated nucleolytic cleavage activity using enzyme reconstituted in a rabbit reticulocyte lysate in vitro transcription/translation system and native enzyme extracted from cells. We found that telomerase catalyzed the removal of nucleotides from DNA substrates including those that can form a mismatch with the RNA template or that contain nontelomeric sequences located 3' to a telomeric sequence. Unlike Tetrahymena telomerase, human telomerase catalyzed the removal of more than one nucleotide (up to 13) from telomeric primers. DNA substrates predicted to align at the 3'-end of the RNA template were not cleaved, consistent with cleavage being dictated by the template 5'-end. We also found some differences in the nuclease activity between RRL-reconstituted human telomerase and native enzyme.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.