ArticleThe Journal of molecular diagnostics : JMD2004
Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay.
Article in The Journal of molecular diagnostics : JMD, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- CanID-PCR: a quick and low-cost PCR tool to identifyMicrobiology spectrum · 2026Article
- Time-to-positivity in bloodstream infection for Candida species as a prognostic marker for mortality.Medical mycology · 2023Article
- Multicenter Evaluation of a PCR-Based Digital Microfluidics and Electrochemical Detection System for the Rapid Identification of 15 Fungal Pathogens Directly from Positive Blood Cultures.Journal of clinical microbiology · 2020Article
- Article
- Time to blood culture positivity as a predictor of clinical outcome in patients with Candida albicans bloodstream infection.BMC infectious diseases · 2013Article
- A quick and low-cost PCR-based assay for Candida spp. identification in positive blood culture bottles.BMC infectious diseases · 2013Article
- Quantification and optimization of Candida albicans DNA in blood samples using Real- Time PCR.Reports of biochemistry & molecular biology · 2013Article
- Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Article
- Quantitation of Candida CFU in initial positive blood cultures.Journal of clinical microbiology · 2011Article
- Rapid detection of blaKPC carbapenemase genes by internally controlled real-time PCR assay using bactec blood culture bottles.Journal of clinical microbiology · 2011Article
- Effect of antifungal therapy timing on mortality in cancer patients with candidemia.Antimicrobial agents and chemotherapy · 2010Article
- Identification of fungal species by fragment length analysis of the internally transcribed spacer 2 region.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.Journal of clinical microbiology · 2009Article
- The 'PREXCEL-Q Method' for qPCR.International journal of biomedical science : IJBS · 2008Article
- Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.Journal of clinical microbiology · 2008Article
- Rapid identification of pathogenic yeast isolates by real-time PCR and two-dimensional melting-point analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.Current genomics · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Delaying the empiric treatment of candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality.Antimicrobial agents and chemotherapy · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a recent study, Candida species in clinical blood samples were detected using a real-time PCR-based method (Maaroufi et al, J Clin Microbiol 2003, 41:3293-3298). For the present study, we evaluated the efficiency of this method as an adjunct to the BACTEC blood culture system to early detection of positivity and negativity of simulated low candidemias. We first established an in vitro correlation between the inoculum of the most frequently encountered Candida species and the time to positivity of these microorganisms. Then, aliquots from blood culture bottles infected with a final average candidal inoculum of 3.18 colony-forming units (CFU)/culture bottle (range, 1 to 6 CFU) were collected at increasing incubation times, and DNA was extracted and submitted to the TaqMan-based PCR assay. To optimize this assay, we evaluated the effect of adding 0.5% bovine serum albumin (BSA) to DNA extracts and found that it decreased the effects of inhibitors. Using specific probes for the tested Candida species, the PCR assay was positive on blood culture aliquots collected from the BACTEC system after a minimum culture turnaround time (TAT) of 3.11 +/- 1.24 hours. Addition of BSA to PCR reaction mixtures improves the TAT (1.84 +/- 0.41 hours). Hence, the combination of DNA "amplification" in the culture bottles by normal growth with an additional DNA amplification by PCR might be a reliable tool facilitating the early diagnosis of low candidemias.
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