ArticleThe Biochemical journal2004
Complementation of the yeast deletion mutant DeltaNCE103 by members of the beta class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity UsingPlants (Basel, Switzerland) · 2022Article
- Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors.AMB Express · 2018Article
- COCurrent genetics · 2017Review
- Carbon Dioxide "Trapped" in a β-Carbonic Anhydrase.Biochemistry · 2015Article
- Acetate regulation of spore formation is under the control of the Ras/cyclic AMP/protein kinase A pathway and carbon dioxide in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Molecular and biochemical analysis of the α class carbonic anhydrases in Caenorhabditis elegans.Molecular biology reports · 2011Article
- A multi-level study of recombinant Pichia pastoris in different oxygen conditions.BMC systems biology · 2010Article
- Article
- Molecular and biochemical analysis of the beta class carbonic anhydrases in Caenorhabditis elegans.Molecular biology reports · 2010Article
- Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103.BMC structural biology · 2009Article
- Article
- The search for the salicylic acid receptor led to discovery of the SAR signal receptor.Plant signaling & behavior · 2008Article
- Carbonic anhydrase (Nce103p): an essential biosynthetic enzyme for growth of Saccharomyces cerevisiae at atmospheric carbon dioxide pressure.The Biochemical journal · 2005Article
- Genome-wide transcription profiling of the early phase of biofilm formation by Candida albicans.Eukaryotic cell · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, members of the beta class of CAs (carbonic anhydrases) have been shown to complement Delta NCE103, a yeast strain unable to grow under aerobic conditions. The activity required for complementation of Delta NCE103 by tobacco chloroplast CA was studied by site-directed mutagenesis. E196A (Glu196-->Ala), a mutated tobacco CA with low levels of CA activity, complemented Delta NCE103. To determine whether restoration of Delta NCE103 was due to residual levels of CA activity or whether it was related to previously proposed antioxidant activity of CAs [Götz, Gnann and Zimmermann (1999) Yeast 15, 855-864], additional complementation analysis was performed using human CAII, an alpha CA structurally unrelated to the beta class of CAs to which the tobacco protein belongs. Human CAII complemented Delta NCE103, strongly arguing that CA activity is responsible for the complementation of Delta NCE103. Consistent with this conclusion, recombinant NCE103 synthesized in Escherichia coli shows CA activity, and Delta NCE103 expressing the tobacco chloroplast CA exhibits the same sensitivity to H2O2 as the wild-type strain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.