ArticlePLoS biology2004
Mimotopes for alloreactive and conventional T cells in a peptide-MHC display library.
Article in PLoS biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 74 citations in OpenAlex.
- Design of solubly expressed miniaturized SMART MHCs.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Aire mediates tolerance to insulin through thymic trimming of high-affinity T cell clones.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Antigen identification and high-throughput interaction mapping by reprogramming viral entry.Nature methods · 2022Article
- Immunogenic self-peptides - the great unknowns in autoimmunity: Identifying T-cell epitopes driving the autoimmune response in autoimmune diseases.Frontiers in immunology · 2022Review
- T cell antigen discovery.Nature methods · 2021Review
- Lysosomal cathepsin creates chimeric epitopes for diabetogenic CD4 T cells via transpeptidation.The Journal of experimental medicine · 2021Article
- Antigen discovery tools for adaptive immune receptor repertoire research.Current opinion in systems biology · 2020Article
- Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo.eLife · 2020Article
- Article
- Stress-testing the relationship between T cell receptor/peptide-MHC affinity and cross-reactivity using peptide velcro.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- N-terminal additions to the WE14 peptide of chromogranin A create strong autoantigen agonists in type 1 diabetes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Baculovirus display of functional antibody Fab fragments.Cytotechnology · 2015Article
- Effect of CDR3 sequences and distal V gene residues in regulating TCR-MHC contacts and ligand specificity.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Article
- Improving antigenic peptide vaccines for cancer immunotherapy using a dominant tumor-specific T cell receptor.The Journal of biological chemistry · 2013Article
- Individual MHCI-Restricted T-Cell Receptors are Characterized by a Unique Peptide Recognition Signature.Frontiers in immunology · 2013Article
- Baculovirus superinfection: a probable restriction factor on the surface display of proteins for library screening.PloS one · 2013Article
- T-cell receptor (TCR) interaction with peptides that mimic nickel offers insight into nickel contact allergy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Diversity-oriented approaches for interrogating T-cell receptor repertoire, ligand recognition, and function.Immunological reviews · 2012Review
- TCR hypervariable regions expressed by T cells that respond to effective tumor vaccines.Cancer immunology, immunotherapy : CII · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The use of peptide libraries for the identification and characterization of T cell antigen peptide epitopes and mimotopes has been hampered by the need to form complexes between the peptides and an appropriate MHC molecule in order to construct a complete T cell ligand. We have developed a baculovirus-based peptide library method in which the sequence encoding the peptide is embedded within the genes for the MHC molecule in the viral DNA, such that insect cells infected with virus encoding a library of different peptides each displays a unique peptide-MHC complex on its surface. We have fished in such a library with two different fluorescent soluble T cell receptors (TCRs), one highly peptide specific and the other broadly allo-MHC specific and hypothesized to be much less focused on the peptide portion of the ligand. A single peptide sequence was selected by the former alphabetaTCR that, not unexpectedly, was highly related to the immunizing peptide. As hypothesized, the other alphabetaTCR selected a large family of peptides, related only by a similarity to the immunizing peptide at the p5 position. These findings have implications for the relative importance of peptide and MHC in TCR ligand recognition. This display method has broad applications in T cell epitope identification and manipulation and should be useful in general in studying interactions between complex proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.