ArticleMolecular biology of the cell2004
Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Cell type specific transcriptional reprogramming of maize leaves during Ustilago maydis induced tumor formation.Scientific reports · 2019Article
- The P5A ATPase Spf1p is stimulated by phosphatidylinositol 4-phosphate and influences cellular sterol homeostasis.Molecular biology of the cell · 2019Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Structural basis for assembly of the CBF3 kinetochore complex.The EMBO journal · 2018Article
- Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.Genetics · 2015Article
- Review
- Dynamic nucleotide-dependent interactions of cysteine- and histidine-rich domain (CHORD)-containing Hsp90 cochaperones Chp-1 and melusin with cochaperones PP5 and Sgt1.The Journal of biological chemistry · 2013Article
- A prescription for 'stress'--the role of Hsp90 in genome stability and cellular adaptation.Trends in cell biology · 2012Review
- Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment.Molecular and cellular biology · 2012Article
- Whole chromosome aneuploidy: big mutations drive adaptation by phenotypic leap.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.The Journal of biological chemistry · 2012Article
- Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation.Molecular biology of the cell · 2011Article
- Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake.The Journal of cell biology · 2011Article
- Scm3 is a centromeric nucleosome assembly factor.The Journal of biological chemistry · 2011Article
- Hsp90-Sgt1 and Skp1 target human Mis12 complexes to ensure efficient formation of kinetochore-microtubule binding sites.The Journal of cell biology · 2010Article
- Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization.The EMBO journal · 2009Article
- Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes.The EMBO journal · 2008Article
- A Bir1p Sli15p kinetochore passenger complex regulates septin organization during anaphase.Molecular biology of the cell · 2007Article
- Positive and negative regulation of Tetrahymena telomerase holoenzyme.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetochores are composed of a large number of protein complexes that must be properly assembled on DNA to attach chromosomes to the mitotic spindle and to coordinate their segregation with the advance of the cell cycle. CBF3 is an inner kinetochore complex in the budding yeast Saccharomyces cerevisiae that nucleates the recruitment of all other kinetochore proteins to centromeric DNA. Skp1p and Sgt1p act through the core CBF3 subunit, Ctf13p, and are required for CBF3 to associate with centromeric DNA. To investigate the contribution of Skp1p and Sgt1p to CBF3 function, we have used a combination of in vitro binding assays and a unique protocol for synchronizing the assembly of kinetochores in cells. We have found that the interaction between Skp1p and Sgt1p is critical for the assembly of CBF3 complexes. CBF3 assembly is not restricted during the cell cycle and occurs in discrete steps; Skp1p and Sgt1p contribute to a final, rate-limiting step in assembly, the binding of the core CBF3 subunit Ctf13p to Ndc10p. The assembly of CBF3 is opposed by its turnover and disruption of this balance compromises kinetochore function without affecting kinetochore formation on centromeric DNA.
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