ArticleMolecular biology of the cell2004
The transcriptional response to Raf activation is almost completely dependent on Mitogen-activated Protein Kinase Kinase activity and shows a major autocrine component.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 71 citations in OpenAlex.
- Targeting the MAPK Pathway in Cancer.International journal of molecular sciences · 2025Review
- Anaplastic thyroid cancer: Genetic roles, targeted therapy, and immunotherapy.Genes & diseases · 2025Review
- Stimulus-Transcription Coupling of TRPM3 Channels: A Signaling Pathway from the Plasma Membrane to the Nucleus.Biomolecules · 2025Review
- Targeting the RAS/RAF/MAPK pathway for cancer therapy: from mechanism to clinical studies.Signal transduction and targeted therapy · 2023Review
- Therapeutic Advancements Across Clinical Stages in Melanoma, With a Focus on Targeted Immunotherapy.Frontiers in oncology · 2021Review
- A phase 1 dose-escalation and expansion study of binimetinib (MEK162), a potent and selective oral MEK1/2 inhibitor.British journal of cancer · 2017Article
- An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer.Nature communications · 2017Article
- Polyisoprenylated cysteinyl amide inhibitors induce caspase 3/7- and 8-mediated apoptosis and inhibit migration and invasion of metastatic prostate cancer cells.American journal of cancer research · 2017Article
- Network Architecture Predisposes an Enzyme to Either Pharmacologic or Genetic Targeting.Cell systems · 2016Article
- Tumor cells with KRAS or BRAF mutations or ERK5/MAPK7 amplification are not addicted to ERK5 activity for cell proliferation.Cell cycle (Georgetown, Tex.) · 2016Article
- Effects of RAL signal transduction in KRAS- and BRAF-mutated cells and prognostic potential of the RAL signature in colorectal cancer.Oncotarget · 2015Article
- Therapeutic control and resistance of the EGFR-driven signaling network in glioblastoma.Cell communication and signaling : CCS · 2015Review
- Loss of EGFR signaling regulated miR-203 promotes prostate cancer bone metastasis and tyrosine kinase inhibitors resistance.Oncotarget · 2014Article
- Mapping the molecular determinants of BRAF oncogene dependence in human lung cancer.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Ultrasonic bioreactor as a platform for studying cellular response.Tissue engineering. Part C, Methods · 2013Article
- Signaling in cell differentiation and morphogenesis.Cold Spring Harbor perspectives in biology · 2012Review
- Raf kinases in cancer-roles and therapeutic opportunities.Oncogene · 2011Review
- An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinomas.American journal of cancer research · 2011Article
- Raf-1 activation prevents caspase 9 processing downstream of apoptosome formation.Journal of signal transduction · 2011Article
- An ERK-dependent pathway to Noxa expression regulates apoptosis by platinum-based chemotherapeutic drugs.Oncogene · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Raf protein kinases are major effectors of Ras GTPases and key components of the transcriptional response to serum factors, acting at least in part through the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway. It has recently been suggested that Raf also may trigger other as yet uncharacterized signaling pathways. Here, we have used cDNA microarrays to dissect changes in gene expression induced by activation of inducible c-Raf-1 constructs in human mammary epithelial and ovarian epithelial cells. The majority of Raf-induced transcriptional responses are shown to be blocked by pharmacological inhibition of the Raf substrate mitogen-activated protein kinase kinase, indicating that potential mitogen-activated protein kinase kinase-independent Raf signaling pathways have no significant influence on gene expression. In addition, we used epidermal growth factor receptor inhibitory drugs to address the contribution of autocrine signaling by Raf-induced EGF family proteins to the Raf transcriptional response. At least one-half of the transcription induced by Raf activation requires epidermal growth factor (EGF) receptor function The EGF receptor-independent component of the Raf transcriptional response is entirely up-regulation of gene expression, whereas the EGF receptor-dependent component is an equal mixture of up- and down-regulation. The use of transcriptional profiling in this way allows detailed analysis of the architecture of signaling pathways to be undertaken.
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