ArticleJournal of bacteriology2004
Identification of PimR as a positive regulator of pimaricin biosynthesis in Streptomyces natalensis.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 125 citations in OpenAlex.
- Abundant organic nitrogen enhances natamycin biosynthesis by increasing NAD(P) metabolic pathway activity inFrontiers in microbiology · 2025Article
- Novel mechanism of hydrogen peroxide for promoting efficient natamycin synthesis inMicrobiology spectrum · 2023Article
- Identification of RimR2 as a positive pathway-specific regulator of rimocidin biosynthesis in Streptomyces rimosus M527.Microbial cell factories · 2023Article
- MilR3, a unique SARP family pleiotropic regulator in Streptomyces bingchenggensis.Archives of microbiology · 2022Article
- Genome Sequence-Guided Finding of Lucensomycin Production byMicroorganisms · 2021Article
- Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA.Scientific reports · 2021Article
- The Application of Regulatory Cascades inFrontiers in microbiology · 2020Review
- Actinomycete-Derived Polyketides as a Source of Antibiotics and Lead Structures for the Development of New Antimicrobial Drugs.Antibiotics (Basel, Switzerland) · 2019Review
- Characterization and overproduction of cell-associated cholesterol oxidase ChoD from Streptomyces lavendulae YAKB-15.Scientific reports · 2019Article
- Challenges and opportunities for natural product discovery, production, and engineering in native producers versus heterologous hosts.Journal of industrial microbiology & biotechnology · 2019Review
- Characterization of the positive SARP family regulator PieR for improving piericidin A1 production inSynthetic and systems biotechnology · 2019Article
- Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges.Synthetic and systems biotechnology · 2018Review
- Promoter Engineering Reveals the Importance of Heptameric Direct Repeats for DNA Binding by Streptomyces Antibiotic Regulatory Protein-Large ATP-Binding Regulator of the LuxR Family (SARP-LAL) Regulators in Streptomyces natalensis.Applied and environmental microbiology · 2018Article
- Identification ofFrontiers in microbiology · 2018Article
- Bidirectional Regulation of AdpAFrontiers in microbiology · 2018Article
- Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02.Scientific reports · 2017Article
- Regulatory genes and their roles for improvement of antibiotic biosynthesis in Streptomyces.3 Biotech · 2017Review
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Overproduction of lactimidomycin by cross-overexpression of genes encoding Streptomyces antibiotic regulatory proteins.Applied microbiology and biotechnology · 2016Article
- Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation.Applied microbiology and biotechnology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was found to have amino acid sequence homology with bacterial regulatory proteins. Database comparisons revealed that PimR represents the archetype of a new class of regulators, combining a Streptomyces antibiotic regulatory protein (SARP)-like N-terminal section with a C-terminal half homologous to guanylate cyclases and large ATP-binding regulators of the LuxR family. Gene replacement of pimR from Streptomyces natalensis chromosome results in a complete loss of pimaricin production, suggesting that PimR is a positive regulator of pimaricin biosynthesis. Gene expression analysis by reverse transcriptase PCR (RT-PCR) of the pimaricin gene cluster revealed that S. natalensis DeltaPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, thus demonstrating that this regulator activates the transcription of all the genes belonging to the pimaricin gene cluster but not its own transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.