ArticleNucleic acids research2004
Real-time PCR genotyping using displacing probes.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Highly sensitive detection of miRNA based on a quencher-free fluorescence strategy with dual-signal amplification.Analytical and bioanalytical chemistry · 2025Article
- ZnO Nanolower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens.Pathogens (Basel, Switzerland) · 2020Article
- Universally applicable, quantitative PCR method utilizing fluorescent nucleobase analogs.RSC advances · 2018Article
- Circulating tumor DNA in patients with colorectal adenomas: assessment of detectability and genetic heterogeneity.Cell death & disease · 2018Article
- Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review.World journal of gastroenterology · 2016Review
- Seven novel probe systems for real-time PCR provide absolute single-base discrimination, higher signaling, and generic components.The Journal of molecular diagnostics : JMD · 2014Article
- Effect of the Bacillus atrophaeus subsp. globigii Spo0F H101R mutation on strain fitness.Applied and environmental microbiology · 2012Article
- Use of duplex mutation primers for real-time PCR quantification of hepatitis C virus RNA in serum.Hepatitis monthly · 2011Article
- Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR.Nucleic acids research · 2007Article
- Algorithm for automatic genotype calling of single nucleotide polymorphisms using the full course of TaqMan real-time data.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple and reliable genotyping technology is a key to success for high-throughput genetic screening in the post-genome era. Here we have developed a new real-time PCR genotyping approach that uses displacement hybridization-based probes: displacing probes. The specificity of displacing probes could be simply assessed through denaturation analysis before genotyping was implemented, and the probes designed with maximal specificity also showed the greatest detection sensitivity. The ease in design, the simple single-dye labeling chemistry and the capability to adopt degenerated negative strands for point mutation genotyping make the displacing probes both cost effective and easy to use. The feasibility of this method was first tested by detecting the C282Y mutation in the human hemochromatosis gene. The robustness of this approach was then validated by simultaneous genotyping of five different types of mutation in the human beta-globin gene. Sixty-two human genomic DNA samples with nine known genotypes were accurately detected, 32 random clinical samples were successfully screened and 114 double-blind DNA samples were all correctly genotyped. The combined merits of reliability, flexibility and simplicity should make this method suitable for routine clinical testing and large-scale genetic screening.
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Registered trials
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