ArticleThe EMBO journal2004
Poly(A)-binding-protein-mediated regulation of hDcp2 decapping in vitro.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- N4BP1 uses tandem KH domains to associate with EDC4 and mRNA decapping factors in P-bodies.Communications biology · 2026Article
- Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.Nature reviews. Molecular cell biology · 2022Review
- 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.PloS one · 2018Article
- A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.The Journal of biological chemistry · 2016Article
- Cap-binding complex (CBC).The Biochemical journal · 2014Review
- miRISC recruits decapping factors to miRNA targets to enhance their degradation.Nucleic acids research · 2013Article
- Multiple Nudix family proteins possess mRNA decapping activity.RNA (New York, N.Y.) · 2013Article
- The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation.Molecular biology of the cell · 2013Article
- The role of mammalian poly(A)-binding proteins in co-ordinating mRNA turnover.Biochemical Society transactions · 2012Review
- Multiple mRNA decapping enzymes in mammalian cells.Molecular cell · 2010Article
- Tethering of poly(A)-binding protein interferes with non-translated mRNA decay from the 5' end in yeast.The Journal of biological chemistry · 2010Article
- Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex.RNA (New York, N.Y.) · 2009Article
- Mutational analysis of a Dcp2-binding element reveals general enhancement of decapping by 5'-end stem-loop structures.Nucleic acids research · 2009Article
- lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo.RNA (New York, N.Y.) · 2008Article
- Inhibition of tristetraprolin deadenylation by poly(A) binding protein.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- DcpS scavenger decapping enzyme can modulate pre-mRNA splicing.RNA (New York, N.Y.) · 2008Article
- Transcript-specific decapping and regulated stability by the human Dcp2 decapping protein.Molecular and cellular biology · 2008Article
- 3' Terminal oligo U-tract-mediated stimulation of decapping.RNA (New York, N.Y.) · 2007Article
- Human TOB, an antiproliferative transcription factor, is a poly(A)-binding protein-dependent positive regulator of cytoplasmic mRNA deadenylation.Molecular and cellular biology · 2007Article
- The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs.RNA (New York, N.Y.) · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of mRNA decapping is a critical determinant for gene expression. We demonstrate that the poly(A) tail-mediated regulation of mRNA decapping observed in humans can be recapitulated in vitro by the cytoplasmic poly(A)-binding protein PABP through a direct and specific binding to the 5' end of capped mRNA. The specific association of PABP with the cap occurred only within the context of the RNA whereby a cap attached to an RNA moiety served as the high-affinity substrate but not the cap structure or RNA alone. Binding of PABP to the RNA 5' end required the presence of the cap and was accentuated by the N7 methyl moiety of the cap. Interestingly, conditions that enhanced hDcp2 decapping activity reduced the affinity of PABP for cap association and consequently its ability to inhibit decapping, suggestive of a regulated association of PABP with the cap. These observations reveal a novel direct involvement of human PABP in the stabilization of mRNA by protecting the 5' end from decapping.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.