ArticleProceedings of the National Academy of Sciences of the United States of America2004
The Arabidopsis repressor of light signaling, COP1, is regulated by nuclear exclusion: mutational analysis by bioluminescence resonance energy transfer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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Who cites it
52 citing papers in PubMed, 130 citations in OpenAlex.
- Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization.Plant, cell & environment · 2026Article
- Spatiotemporal regulation of anther's tapetum degeneration paved the way for a reversible male sterility system in cotton.Plant biotechnology journal · 2025Article
- Revisiting the role of light signaling in plant responses to salt stress.Horticulture research · 2025Article
- Light signaling in plants-a selective history.Plant physiology · 2024Review
- Phytochrome phosphorylation in plant light signaling.Frontiers in plant science · 2024Review
- Shade avoidance in the context of climate change.Plant physiology · 2023Article
- Regulation of Plant Photoresponses by Protein Kinase Activity of Phytochrome A.International journal of molecular sciences · 2023Review
- Overexpression of a pseudo-etiolated-in-light-like protein inFrontiers in plant science · 2023Article
- COP1 dynamics integrate conflicting seasonal light and thermal cues in the control ofScience advances · 2022Article
- Light Induced Regulation Pathway of Anthocyanin Biosynthesis in Plants.International journal of molecular sciences · 2021Review
- Constitutive Photomorphogenic 1 Enhances ER Stress Tolerance in Arabidopsis.International journal of molecular sciences · 2021Article
- Phytochrome Signaling Networks.Annual review of plant biology · 2021Review
- Direct phosphorylation of HY5 by SPA kinases to regulate photomorphogenesis in Arabidopsis.The New phytologist · 2021Article
- Out of the Dark and Into the Light: A New View of Phytochrome Photobodies.Frontiers in plant science · 2021Review
- Illuminating the COP1/SPA Ubiquitin Ligase: Fresh Insights Into Its Structure and Functions During Plant Photomorphogenesis.Frontiers in plant science · 2021Review
- PCH1 and PCHL Directly Interact with PIF1, Promote Its Degradation, and Inhibit Its Transcriptional Function during Photomorphogenesis.Molecular plant · 2020Article
- A phyB-PIF1-SPA1 kinase regulatory complex promotes photomorphogenesis in Arabidopsis.Nature communications · 2019Article
- Plant photoreceptors: Multi-functional sensory proteins and their signaling networks.Seminars in cell & developmental biology · 2019Review
- COP1 regulates the stability of CAM7 to promote photomorphogenic growth.Plant direct · 2019Article
- The pseudokinase TRIB1 toggles an intramolecular switch to regulate COP1 nuclear export.The EMBO journal · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bioluminescence resonance energy transfer (BRET) between Renilla luciferase and yellow fluorescent protein has been adapted to serve as a real-time reporter on protein-protein interactions in live plant cells by using the Arabidopsis Constitutive photomorphogenesis 1 (COP1) protein as a model system. COP1 is a repressor of light signal transduction that functions as part of a nuclear E3 ubiquitin ligase. COP1 possesses a leucine-rich nuclear-exclusion signal that resides in a domain implicated in COP1 dimerization. BRET was applied in conjunction with site-directed mutagenesis to explore the respective contributions of the nuclear-exclusion and dimerization motifs to the regulation of COP1 activity in vivo. One specific mutant protein, COP1(L105A), showed increased nuclear accumulation but retained the ability to dimerize, as monitored by BRET, whereas other mutations inhibited both nuclear exclusion and COP1 dimerization. Mutant rescue and overexpression experiments indicated that nuclear exclusion of COP1 protein is a rate-limiting step in light signal transduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.