ArticleProceedings of the National Academy of Sciences of the United States of America2004
Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 276 citations in OpenAlex.
- A Comprehensive Review on the Biosynthesis of Tropane Alkaloids.Molecules (Basel, Switzerland) · 2026Review
- Enhancing the accumulation of scopolamine and atropine in Hyoscyamus niger L. callus by LED light and glycine-a cheap method.BMC plant biology · 2025Article
- A chromosome-level genome assembly of anesthetic drug-producing Anisodus acutangulus provides insights into its evolution and the biosynthesis of tropane alkaloids.Plant communications · 2024Article
- First Report on Mesophyll Protoplast Isolation and Regeneration System for thePlants (Basel, Switzerland) · 2023Article
- Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles' biosynthetic logic.BMC plant biology · 2023Article
- The Plant Virus Tomato Spotted Wilt Orthotospovirus Benefits Its VectorInternational journal of molecular sciences · 2023Article
- The Light- and Jasmonic Acid-InducedInternational journal of molecular sciences · 2023Article
- Systematic approaches to C-lignin engineering in Medicago truncatula.Biotechnology for biofuels and bioproducts · 2023Article
- Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants.Frontiers in plant science · 2023Review
- Biotic Elicitors in Adventitious and Hairy Root Cultures: A Review from 2010 to 2022.Molecules (Basel, Switzerland) · 2022Review
- Unravelling the multi-faceted regulatory role of polyamines in plant biotechnology, transgenics and secondary metabolomics.Applied microbiology and biotechnology · 2022Review
- Biotechnological Approaches on Engineering Medicinal Tropane Alkaloid Production in Plants.Frontiers in plant science · 2022Review
- Activation modes in biocatalytic radical cyclization reactions.Journal of industrial microbiology & biotechnology · 2021Review
- Development ofInternational journal of molecular sciences · 2021Article
- Plant tissue culture as a perpetual source for production of industrially important bioactive compounds.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Regioselectivity of hyoscyamine 6β-hydroxylase-catalysed hydroxylation as revealed by high-resolution structural information and QM/MM calculations.Dalton transactions (Cambridge, England : 2003) · 2020Article
- Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis.PloS one · 2020Article
- AFrontiers in plant science · 2020Article
- Hairy Root Cultures-A Versatile Tool With Multiple Applications.Frontiers in plant science · 2020Review
- Tropane Alkaloids: Chemistry, Pharmacology, Biosynthesis and Production.Molecules (Basel, Switzerland) · 2019Review
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an anticholinergic agent. Here, we report the simultaneous introduction and overexpression of genes encoding the rate-limiting upstream enzyme putrescine N-methyltransferase (PMT) and the downstream enzyme hyoscyamine 6 beta-hydroxylase (H6H) of scopolamine biosynthesis in transgenic henbane (Hyoscyamus niger) hairy root cultures. Transgenic hairy root lines expressing both pmt and h6h produced significantly higher (P < 0.05) levels of scopolamine compared with the wild-type and transgenic lines harboring a single gene (pmt or h6h). The best line (T(3)) produced 411 mg/liter scopolamine, which was over nine times more than that in the wild type (43 mg/liter) and more than twice the amount in the highest scopolamine-producing h6h single-gene transgenic line H(11) (184 mg/liter). To our knowledge, this is the highest scopolamine content achieved through genetic engineering of a plant. We conclude that transgenic plants harboring both pmt and h6h possessed an increased flux in the tropane alkaloid biosynthetic pathway that enhanced scopolamine yield, which was more efficient than plants harboring only one of the two genes. It seems that the pulling force of the downstream enzyme (the faucet enzyme) H6H plays a more important role in stimulating scopolamine accumulation in H. niger whereas the functioning of the upstream enzyme PMT is increased proportionally. This study provides an effective approach for large-scale commercial production of scopolamine by using hairy root culture systems as bioreactors.
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