ArticleNucleic acids research2004
Dual FRET molecular beacons for mRNA detection in living cells.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.
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Who cites it
128 citing papers in PubMed.
- Article
- A Brief Progress in Methods for Deciphering Protein-Protein Interaction Networks.International journal of molecular sciences · 2026Review
- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Long noncoding RNA MALAT1 is dynamically regulated in leader cells during collective cancer invasion.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production.Communications biology · 2023Article
- Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules.Frontiers in bioengineering and biotechnology · 2023Review
- Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.Nucleic acids research · 2022Article
- Review
- Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells.Nucleic acids research · 2022Article
- Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells.Molecular therapy. Nucleic acids · 2022Article
- A "turn-on" DNA-scaffolded silver-nanocluster probe for detection of tumor-related mRNA.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- DNA-enabled fluorescent-based nanosensors monitoring tumor-related RNA toward advanced cancer diagnosis: A review.Frontiers in bioengineering and biotechnology · 2022Review
- Novel nucleic acid origami structures and conventional molecular beacon-based platforms: a comparison in biosensing applications.Analytical and bioanalytical chemistry · 2021Review
- Ion-exchanged glass microrods as hybrid SERS/fluorescence substrates for molecular beacon-based DNA detection.Analytical and bioanalytical chemistry · 2021Article
- Intermolecular dark resonance energy transfer (DRET): upgrading fluorogenic DNA sensing.Nucleic acids research · 2021Article
- Diversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing.The CRISPR journal · 2021Article
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Different Approaches to Develop Nanosensors for Diagnosis of Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
68 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to visualize in real-time the expression level and localization of specific endogenous RNAs in living cells can offer tremendous opportunities for biological and disease studies. Here we demonstrate such a capability using a pair of molecular beacons, one with a donor and the other with an acceptor fluorophore that hybridize to adjacent regions on the same mRNA target, resulting in fluorescence resonance energy transfer (FRET). Detection of the FRET signal significantly reduced false positives, leading to sensitive imaging of K-ras and survivin mRNAs in live HDF and MIAPaCa-2 cells. FRET detection gave a ratio of 2.25 of K-ras mRNA expression in stimulated and unstimulated HDF, comparable to the ratio of 1.95 using RT-PCR, and in contrast to the single-beacon result of 1.2. We further revealed intriguing details of K-ras and survivin mRNA localization in living cells. The dual FRET molecular beacons approach provides a novel technique for sensitive RNA detection and quantification in living cells.
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