ArticleBMC genomics2004
Microarray genomotyping of key experimental strains of Neisseria gonorrhoeae reveals gene complement diversity and five new neisserial genes associated with Minimal Mobile Elements.
Article in BMC genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Diversity of the type VI secretion systems in theMicrobial genomics · 2023Article
- Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages.PLoS pathogens · 2021Article
- DNA uptake sequences inMicrobial genomics · 2016Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- Identification of sRNAs expressed by the human pathogen Neisseria gonorrhoeae under disparate growth conditions.Frontiers in microbiology · 2014Article
- Protocol for gene expression profiling using DNA microarrays in Neisseria gonorrhoeae.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Review and international recommendation of methods for typing neisseria gonorrhoeae isolates and their implications for improved knowledge of gonococcal epidemiology, treatment, and biology.Clinical microbiology reviews · 2011Review
- Evaluation of one- and two-color gene expression arrays for microbial comparative genome hybridization analyses in routine applications.Journal of clinical microbiology · 2010Article
- Comparative analysis of two Neisseria gonorrhoeae genome sequences reveals evidence of mobilization of Correia Repeat Enclosed Elements and their role in regulation.BMC genomics · 2009Article
- Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice.Vaccine · 2007Article
- The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger Escherichia coli FNR regulon and interaction with the NarQ-NarP regulon.BMC genomics · 2007Article
- The majority of genes in the pathogenic Neisseria species are present in non-pathogenic Neisseria lactamica, including those designated as 'virulence genes'.BMC genomics · 2006Article
- Design, validation, and application of a seven-strain Staphylococcus aureus PCR product microarray for comparative genomics.Applied and environmental microbiology · 2005Article
- Strain-specific differences in Neisseria gonorrhoeae associated with the phase variable gene repertoire.BMC microbiology · 2005Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThere are four widely used experimental strains of N. gonorrhoeae, one of which has been sequenced and used as the basis for the construction of a multi-strain, mutli-species pan-neisserial microarray. Although the N. gonorrhoeae population structure is thought to be less diverse than N. meningitidis, there are some recognized gene-complement differences between strains, including the 59 genes of the Gonococcal Genetic Island. In this study we have investigated the three experimental strains that have not been sequenced to determine the extent and nature of their similarities and differences.
resultsUsing the Pan-Neisseria microarray, three commonly used gonococcal laboratory experimental strains were investigated (F62, MS11, & FA19). Genes absent from these strains, but present in strain FA1090, were assessed as is possible with typical microarrays. Due to the design of this microarray, additional genes were also identified. Differences were associated with Minimal Mobile Elements (MMEs) or known divergences. Genomotyping indicates the presence of genes previously only described in meningococci and shows the presence of the complete Gonococcal Genetic Island in N. gonorrhoeae strain FA19. Five new neisserial genes were identified through microarray genomotyping and subsequent sequencing of two divergent MMEs in N. gonorrhoeae strain MS11 and four MMEs in N. gonorrhoeae strain FA19. No differences were identified between N. gonorrhoeae strains FA1090 and F62, indicating that these strains are very similar.
conclusionThis study shows extensive similarity between the experimental strains, associated with a varying number of strain-specific genes. This provides a framework for those working with these strains to refer to the available gonococcal genome sequence, and is the first detailed comparison of gene complements between gonococcal strains.
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