Trial reportBritish journal of cancer2004
Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple mRNA markers.
Trial report in British journal of cancer, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 68 citations in OpenAlex.
- Detection of micrometastases by flow cytometry in sentinel lymph nodes from patients with renal tumours.British journal of cancer · 2016Trial
- Targeting MUCL1 protein inhibits cell proliferation and EMT by deregulating β‑catenin and increases irinotecan sensitivity in colorectal cancer.International journal of oncology · 2022Article
- One-step nucleic acid amplification: the possible value in assessing sentinel lymph node metastasis during mastectomy.Breast cancer (Dove Medical Press) · 2018Review
- Article
- Trefoil Factor-3 (TFF3) Stimulates De Novo Angiogenesis in Mammary Carcinoma both Directly and Indirectly via IL-8/CXCR2.PloS one · 2015Article
- Article
- An improved axillary staging system using the OSNA assay does not modify the therapeutic management of breast cancer patients.Scientific reports · 2014Article
- Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease.PloS one · 2013Article
- Accurate and rapid novel genetic diagnosis for detection of sentinel lymph node metastasis in breast cancer patients.British journal of cancer · 2012Article
- The prognostic significance of tumour cell detection in the peripheral blood versus the bone marrow in 733 early-stage breast cancer patients.Breast cancer research : BCR · 2011Article
- A multimarker QPCR-based platform for the detection of circulating tumour cells in patients with early-stage breast cancer.British journal of cancer · 2011Article
- Intraoperative assessment of sentinel lymph nodes in breast cancer.The British journal of surgery · 2011Review
- Trefoil factor 3 is oncogenic and mediates anti-estrogen resistance in human mammary carcinoma.Neoplasia (New York, N.Y.) · 2010Article
- [Sentinel node biopsy in breast cancer: pathological analysis and interpretation].Der Pathologe · 2009Review
- One-step nucleic acid amplification-a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group.Virchows Archiv : an international journal of pathology · 2009Article
- Intra-operative rapid diagnostic method based on CK19 mRNA expression for the detection of lymph node metastases in breast cancer.International journal of cancer · 2008Article
- Minimal disease in sentinel nodes.Pathology oncology research : POR · 2008Review
- Expression of small breast epithelial mucin (SBEM) protein in tissue microarrays (TMAs) of primary invasive breast cancers.Histopathology · 2008Article
- Intracellular esterase activity in living cells may distinguish between metastatic and tumor-free lymph nodes.Clinical & experimental metastasis · 2008Article
- HRPAP20: a novel calmodulin-binding protein that increases breast cancer cell invasion.Oncogene · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Disseminated breast tumour cells in sentinel lymph nodes (SNs) were evaluated by quantitative real-time PCR and the sensitivity of this assay was compared to the routine histological analysis. First, several candidate marker genes were tested for their specificity in axillary lymph nodes (ALN) of 50 breast cancer patients and 43 women without breast cancer. The marker gene panel selected, designed to detect the mRNA of CK19, p1B, EGP2 and SBEM, was subsequently applied to detect metastases in 70 SNs that were free of metastases as determined by standard histological evaluation. Remarkably, seven negative SNs showed increased marker gene expression, suggesting the presence of (micro) metastases. Four of these seven SNs positive by real-time PCR proved to contain tumour deposits after careful review of the slides or further sectioning of the paraffin-embedded material. In three PCR positive SNs, however, no tumour cells were found by haematoxylin and eosin staining (H&E) and immunohistologically analysis. The quantitative real-time PCR assay with multiple mRNA markers for the detection of disseminated breast cancer cells in SNs thus resulted in an upstaging of SNs containing metastastic disease of 10% compared to the routine histological analysis. The application of this technique may be of clinical relevance, as it is suggested that micrometastatic disease in SNs are associated with further nodal non-SN metastases in breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.