ArticlePlant molecular biology2003
Identification of differentially expressed cDNA sequences in ovaries of sexual and apomictic plants of Brachiaria brizantha.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 64 citations in OpenAlex.
- Identification of IPT9 in Brachiaria brizantha (syn. Urochloa brizantha) and expression analyses during ovule development in sexual and apomictic plants.Molecular biology reports · 2023Article
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- Anther development in Brachiaria brizantha (syn. Urochloa brizantha) and perspective for microspore in vitro culture.Protoplasma · 2023Article
- Diverged subpopulations in tropical Urochloa (Brachiaria) forage species indicate a role for facultative apomixis and varying ploidy in their population structure and evolution.Annals of botany · 2022Article
- An Overview of the Genetics and Genomics of theFrontiers in plant science · 2021Review
- Article
- Article
- Review
- A Plant-SpecificFrontiers in plant science · 2019Article
- GID1 expression is associated with ovule development of sexual and apomictic plants.Plant cell reports · 2018Article
- The MAP3K-CodingFrontiers in plant science · 2018Article
- A reference floral transcriptome of sexual and apomictic Paspalum notatum.BMC genomics · 2017Article
- PnTgs1-like expression during reproductive development supports a role for RNA methyltransferases in the aposporous pathway.BMC plant biology · 2014Article
- Apomictic and sexual germline development differ with respect to cell cycle, transcriptional, hormonal and epigenetic regulation.PLoS genetics · 2014Article
- A conserved apomixis-specific polymorphism is correlated with exclusive exonuclease expression in premeiotic ovules of apomictic boechera species.Plant physiology · 2013Article
- Enlarging cells initiating apomixis in Hieracium praealtum transition to an embryo sac program prior to entering mitosis.Plant physiology · 2013Article
- Apomixis in plant reproduction: a novel perspective on an old dilemma.Plant reproduction · 2013Review
- Expression analyses of Brachiaria brizantha genes encoding ribosomal proteins BbrizRPS8, BbrizRPS15a, and BbrizRPL41 during development of ovaries and anthers.Protoplasma · 2013Article
- Transcriptome analysis of differentially expressed genes during embryo sac development in apomeiotic non-parthenogenetic interspecific hybrid of Pennisetum glaucum.Molecular biotechnology · 2012Article
- Expressed sequence-tag analysis of ovaries of Brachiaria brizantha reveals genes associated with the early steps of embryo sac differentiation of apomictic plants.Plant cell reports · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation of genes associated with apomixis would improve understanding of the molecular mechanism of this mode of reproduction in plants as well as open the possibility of transfer of apomixis to sexual plants, enabling cloning of crops through seeds. Brachiaria brizantha is a highly apomictic grass species with 274 tetraploid apomicts accessions and only one diploid sexual. In this study we have compared gene expression in ovaries at megasporogenesis and megagametogenesis of sexual and apomictic accessions of B. brizantha by differential display (DD-PCR), with 60 primer combinations. Specificity of 65 cloned fragments, checked by reverse northern blot analysis, showed that 11 clones were differentially expressed, 6 in apomictic ovaries, 2 in sexual and 3 in apomictic and sexual, but at different stages. Of the 6 sequences isolated that were preferentially expressed in the apomictic accession: one sequence was from ovaries at megasporogenesis stage; three were from megagametogenesis stage; two were from both stages. Of the two sequences isolated from the sexual accessions, one showed expression in ovaries at megagametogenesis, while the other sequence was shown to be specific to both stages. Three sequences were from megasporogenesis stage in apomicts but were also detected at megagametogenesis in sexual plants. Sequence analysis showed that 5 of the 11 clones had no apparent homologues in the protein database. Some of the clones identified as apomictic-specific shared homology with known genes enabling their functional annotation. The relationships of these functions to the generation of the apomictic trait are discussed.
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