ArticleMolecular and cellular biology2004
The GTPase-activating enzyme Gyp1p is required for recycling of internalized membrane material by inactivation of the Rab/Ypt GTPase Ypt1p.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Arf1 orchestrates Rab GTPase conversion at theMolecular biology of the cell · 2021Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- Gyp1 has a dual function as Ypt1 GAP and interaction partner of Atg8 in selective autophagy.Autophagy · 2019Article
- Autophagy in the context of the cellular membrane-trafficking system: the enigma of Atg9 vesicles.Biochemical Society transactions · 2017Review
- Autophagy in Saccharomyces cerevisiae requires the monomeric GTP-binding proteins, Arl1 and Ypt6.Autophagy · 2016Article
- Suppression of α-synuclein toxicity and vesicle trafficking defects by phosphorylation at S129 in yeast depends on genetic context.Human molecular genetics · 2012Article
- Modular TRAPP complexes regulate intracellular protein trafficking through multiple Ypt/Rab GTPases in Saccharomyces cerevisiae.Genetics · 2012Article
- Peptides induce persistent signaling from endosomes by a nutrient transceptor.Nature chemical biology · 2012Article
- Mutational analysis of the yeast TRAPP subunit Trs20p identifies roles in endocytic recycling and sporulation.PloS one · 2012Article
- GTPase networks in membrane traffic.Annual review of biochemistry · 2012Review
- Intracellular phospholipase A1 and acyltransferase, which are involved in Caenorhabditis elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol.Molecular biology of the cell · 2010Article
- Isolation of a fission yeast mutant that is sensitive to valproic acid and defective in the gene encoding Ric1, a putative component of Ypt/Rab-specific GEF for Ryh1 GTPase.Molecular genetics and genomics : MGG · 2010Article
- Establishing a role for the GTPase Ypt1p at the late Golgi.Traffic (Copenhagen, Denmark) · 2010Article
- Genetic evidence that the higher plant Rab-D1 and Rab-D2 GTPases exhibit distinct but overlapping interactions in the early secretory pathway.Journal of cell science · 2009Article
- A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Identification of a conserved F-box protein 6 interactor essential for endocytosis and cytokinesis in fission yeast.The Biochemical journal · 2009Article
- Phospholipid transfer protein Sec14 is required for trafficking from endosomes and regulates distinct trans-Golgi export pathways.The Journal of biological chemistry · 2009Article
- The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion.Molecular biology of the cell · 2008Article
- Beta-catenin asymmetry is regulated by PLA1 and retrograde traffic in C. elegans stem cell divisions.The EMBO journal · 2008Article
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab/Ypt GTPases are key regulators of membrane trafficking and together with SNARE proteins mediate selective fusion of vesicles with target compartments. A family of GTPase-activating enzymes (GAPs) specific for Rab/Ypt GTPases has been discovered, but little is known about their function and substrate specificity in vivo. Here we show that the GAP activity of Gyp1p, a yeast member of this family, is specifically required for recycling of the SNARE Snc1p and the membrane dye FM4-64, implying that inactivation of a Rab/Ypt GTPase may be necessary for recycling of membrane material. Interestingly, recycling of GFP-Snc1p in gyp1 Delta cells is partially restored by reducing the activity of Ypt1p. Moreover, GFP-Snc1p accumulated intracellularly in wild-type cells expressing a GTP-locked, mutant form of Ypt1p (Ypt1p-Q67L), suggesting that GTP hydrolysis of Ypt1p is essential for recycling. Ypt6p is known to be required for the fusion of recycling vesicles to the late Golgi compartment. Interestingly, the deletions of GYP1 and YPT6 were synthetic lethal, raising the possibility that at least two distinct pathways are involved in recycling of membrane material.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.