ArticleMolecular and cellular biology2004
High-level activation of transcription of the yeast U6 snRNA gene in chromatin by the basal RNA polymerase III transcription factor TFIIIC.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Terminator-dependent facilitated recycling of RNA polymerase III provides gene-specific transcriptional activation in vivo.Science advances · 2026Article
- Structural Features of the Nucleosomal DNA Modulate the Functional Binding of a Transcription Factor and Productive Transcription.Frontiers in genetics · 2022Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Nucleosome Positioning and NDR Structure at RNA Polymerase III Promoters.Scientific reports · 2017Article
- Cytoskeletal Filamin A Differentially Modulates RNA Polymerase III Gene Transcription in Transformed Cell Lines.The Journal of biological chemistry · 2016Article
- Systematic characterization of the conformation and dynamics of budding yeast chromosome XII.The Journal of cell biology · 2013Article
- Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes.Nucleic acids research · 2011Article
- Multiple sequence-directed possibilities provide a pool of nucleosome position choices in different states of activity of a gene.Epigenetics & chromatin · 2009Article
- Chromatin structure and expression of a gene transcribed by RNA polymerase III are independent of H2A.Z deposition.Molecular and cellular biology · 2008Article
- CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.Molecular and cellular biology · 2007Article
- Transcriptional activation of a moderately expressed tRNA gene by a positioned nucleosome.The Biochemical journal · 2006Article
- Modulation of differential transcription of tRNA genes through chromatin organization.The Biochemical journal · 2005Article
- The growing pre-mRNA recruits actin and chromatin-modifying factors to transcriptionally active genes.Genes & development · 2005Article
- Transcription termination by the eukaryotic RNA polymerase III.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Transcription of the U6 snRNA gene (SNR6) in Saccharomyces cerevisiae by RNA polymerase III (pol III) requires TFIIIC and its box A and B binding sites. In contrast, TFIIIC has little or no effect on SNR6 transcription with purified components in vitro due to direct recognition of the SNR6 TATA box by TFIIIB. When SNR6 was assembled into chromatin in vitro by use of the Drosophila melanogaster S-190 extract, transcription of these templates with highly purified yeast pol III, TFIIIC, and TFIIIB displayed a near-absolute requirement for TFIIIC but yielded a 5- to 15-fold-higher level of transcription relative to naked DNA (>100-fold activation over repressed chromatin). Analysis of chromatin structure demonstrated that TFIIIC binding leads to remodeling of U6 gene chromatin, resulting in positioning of a nucleosome between boxes A and B. The resulting folding of the intervening DNA into the nucleosome could bring the suboptimally spaced SNR6 box A and B elements into greater proximity and thus facilitate activation of transcription. In the absence of ATP, however, the binding of TFIIIC to box B in chromatin was not accompanied by remodeling and the transcription activation was approximately 35% of that seen in its presence, implying that both TFIIIC binding and ATP-dependent chromatin remodeling were required for the full activation of the gene. Our results suggest that TFIIIC, which is a basal transcription factor of pol III, also plays a direct role in remodeling chromatin on the SNR6 gene.
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