ArticleGut2004
Pro-hepcidin: expression and cell specific localisation in the liver and its regulation in hereditary haemochromatosis, chronic renal insufficiency, and renal anaemia.
Article in Gut, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 247 citations in OpenAlex.
- Effect of daprodustat on anemia in patients with chronic kidney disease: a meta-analysis.International urology and nephrology · 2018Pooled it
- Serum levels of the hepcidin-20 isoform in a large general population: the Val Borbera study.Journal of proteomics · 2012Trial
- Oxpentifylline versus placebo in the treatment of erythropoietin-resistant anaemia: a randomized controlled trial.BMC nephrology · 2008Trial
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- Conventional and complementary alternative medicine therapies for renal anemia: a literature review.Frontiers in endocrinology · 2024Review
- Host and Pathogen-Directed Therapies against Microbial Infections Using Exosome- and Antimicrobial Peptide-derived Stem Cells with a Special look at Pulmonary Infections and Sepsis.Stem cell reviews and reports · 2023Review
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- Potential of Mesenchymal Stem Cell-Derived Exosomes as a Novel Treatment for Female Infertility Caused by Bacterial Infections.Frontiers in microbiology · 2021Review
- Hepcidin and GDF-15 are potential biomarkers of iron deficiency anaemia in chronic kidney disease patients in South Africa.BMC nephrology · 2020Article
- TGFβ2-Hepcidin Feed-Forward Loop in the Trabecular Meshwork Implicates Iron in Glaucomatous Pathology.Investigative ophthalmology & visual science · 2020Article
- Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications.Experimental eye research · 2020Article
- Is hepcidin related with anemia and bone mineral metabolism in children with non-dialysis chronic kidney disease?Turk pediatri arsivi · 2019Article
- Iron Regulation: Macrophages in Control.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Low hepcidin in liver fibrosis and cirrhosis; a tale of progressive disorder and a case for a new biochemical marker.Molecular medicine (Cambridge, Mass.) · 2018Review
- Normalizing serum hepcidin but not α-1-antitrypsin level during effective treatment of chronic hepatitis C.Clinical and experimental hepatology · 2017Article
- Expression of ayu antimicrobial peptide genes after LPS stimulation.The Journal of veterinary medical science · 2017Article
- Laboratory use of hepcidin in renal transplant recipients.Biochemia medica · 2016Review
- An insight into the relationships between prohepcidin, iron deficiency anemia, and interleukin-6 values in pediatric Helicobacter pylori gastritis.European journal of pediatrics · 2015Article
- Serum prohepcidin levels are lower in patients with atrophic gastritis.Gastroenterology research and practice · 2013Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsThe hepatic peptide hormone hepcidin, which has recently been isolated from human plasma and urine, is thought to be a central regulator of iron homeostasis. We investigated the presence and cellular localisation of hepcidin in the liver and developed a non-invasive assay to analyse its regulation in patients with hereditary haemochromatosis (HH), chronic renal insufficiency (CRI), and renal anaemia (RA).
methodsExpression and localisation of hepcidin was shown by reverse transcription-polymerase chain reaction, western blot, immunocytochemistry, and immunofluorescence in human and guinea pig liver. Serum concentrations were determined in various groups of patients using a sensitive enzyme linked immunosorbent assay (ELISA).
resultsWestern blot analysis with region specific antibodies identified a approximately 10 kDa peptide corresponding to the apparent molecular mass of pro-hepcidin. Localisation studies revealed that pro-hepcidin is expressed at the basolateral membrane domain of hepatocytes and is also present in blood. We developed a stable sensitive ELISA for detection and determination of pro-hepcidin in human serum. Mean pro-hepcidin level in human serum of healthy volunteers was 106.2 ng/ml. Enhanced levels of pro-hepcidin (148.1 ng/ml) were found in patients with CRI but normal haemoglobin values, indicating that the kidneys may metabolise and/or eliminate the circulating hormone. In contrast, concentrations of pro-hepcidin were significantly decreased in patients with HH (70.2 ng/ml) and also in patients with RA (115.0 ng/ml) compared with the CRI group.
conclusionsFrom the detection of pro-hepcidin in human serum, we conclude that the prohormone may be involved in the regulation of iron metabolism in HH. Decreased pro-hepcidin levels could play an important role in the pathogenesis of HH.
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