Evidence map›Paper›PMID 15082563›Full record

ArticleGenetics2004

A sequence-based genetic map of Medicago truncatula and comparison of marker colinearity with M. sativa.

Hong-Kyu Choi, Dongjin Kim, Taesik Uhm, Eric Limpens, Hyunju Lim, Jeong-Hwan Mun, Peter Kalo, R Varma Penmetsa, Andrea Seres, Olga Kulikova and 4 more

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.

0numbers the graph read from it
0cells of the map it votes in
113citing papers in PubMed
31.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

113 citing papers in PubMed, 326 citations in OpenAlex.

  1. PhytoKeys · 2025
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  3. Speciation and evolution of growth form inFrontiers in plant science · 2024
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  5. Review
  6. OMICS in Fodder Crops: Applications, Challenges, and Prospects.Current issues in molecular biology · 2022
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Genomics of Plant Disease Resistance in Legumes.Frontiers in plant science · 2019
    Review
  12. Article
  13. First genetic linkage map ofHorticulture research · 2018
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Increasing seed size and quality by manipulating BIG SEEDS1 in legume species.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article

53 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Hong-Kyu ChoiDepartment of Plant Pathology, University of California, Davis, California 95616, USA.
Dongjin Kim
Taesik Uhm
Eric Limpens
Hyunju Lim
Jeong-Hwan Mun
Peter Kalo
R Varma Penmetsa
Andrea Seres
Olga Kulikova
Bruce A Roe
Ton Bisseling
Gyorgy B Kiss
Douglas R Cook
University of California, Davis · USHUN-REN Szegedi Biológiai Kutatóközpont · HUTexas A&M University · USInstitute of Genetics · HUUniversity of Oklahoma · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A core genetic map of the legume Medicago truncatula has been established by analyzing the segregation of 288 sequence-characterized genetic markers in an F(2) population composed of 93 individuals. These molecular markers correspond to 141 ESTs, 80 BAC end sequence tags, and 67 resistance gene analogs, covering 513 cM. In the case of EST-based markers we used an intron-targeted marker strategy with primers designed to anneal in conserved exon regions and to amplify across intron regions. Polymorphisms were significantly more frequent in intron vs. exon regions, thus providing an efficient mechanism to map transcribed genes. Genetic and cytogenetic analysis produced eight well-resolved linkage groups, which have been previously correlated with eight chromosomes by means of FISH with mapped BAC clones. We anticipated that mapping of conserved coding regions would have utility for comparative mapping among legumes; thus 60 of the EST-based primer pairs were designed to amplify orthologous sequences across a range of legume species. As an initial test of this strategy, we used primers designed against M. truncatula exon sequences to rapidly map genes in M. sativa. The resulting comparative map, which includes 68 bridging markers, indicates that the two Medicago genomes are highly similar and establishes the basis for a Medicago composite map.

Indexed as

Chromosome MappingBase SequenceChromosomes, Artificial, BacterialChromosomes, PlantCytogenetic AnalysisDNA, PlantExpressed Sequence TagsGenetic LinkageGenetic MarkersGenome, PlantIn Situ Hybridization, FluorescenceIntronsMedicagoMedicago sativaPolymorphism, GeneticTranscription, GeneticDNA, PlantGenetic Markers

Identifiers

PMID15082563
PMCPMC1470769
OpenAlexW2103714924

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.