ArticleGenetics2004
Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Histone variant H2A.Z is needed for efficient transcription-coupled NER and genome integrity in UV challenged yeast cells.PLoS genetics · 2024Article
- Swi/Snf chromatin remodeling regulates transcriptional interference and gene repression.Molecular cell · 2024Article
- Swi/Snf Chromatin Remodeling Regulates Transcriptional Interference and Gene Repression.bioRxiv : the preprint server for biology · 2023Article
- Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transcription fidelity: New paradigms in epigenetic inheritance, genome instability and disease.DNA repair · 2019Review
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- RNA Polymerase II Trigger Loop Mobility: INDIRECT EFFECTS OF Rpb9.The Journal of biological chemistry · 2016Article
- The prefoldin complex regulates chromatin dynamics during transcription elongation.PLoS genetics · 2013Article
- TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress.Nucleic acids research · 2012Article
- Isolation and characterization of transcription fidelity mutants.Biochimica et biophysica acta · 2012Review
- Role of Mediator in regulating Pol II elongation and nucleosome displacement in Saccharomyces cerevisiae.Genetics · 2012Article
- Understanding and predicting synthetic lethal genetic interactions in Saccharomyces cerevisiae using domain genetic interactions.BMC systems biology · 2011Article
- Histone variant H2A.Z and RNA polymerase II transcription elongation.Molecular and cellular biology · 2011Article
- The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation.Nature reviews. Genetics · 2010Review
- Overexpression of SNG1 causes 6-azauracil resistance in Saccharomyces cerevisiae.Current genetics · 2010Article
- The YEATS domain of Taf14 in Saccharomyces cerevisiae has a negative impact on cell growth.Molecular genetics and genomics : MGG · 2010Article
- Site-directed mutagenesis, purification and assay of Saccharomyces cerevisiae RNA polymerase II.Protein expression and purification · 2010Article
- The basal initiation machinery: beyond the general transcription factors.Current opinion in cell biology · 2009Review
- Identification, structure, and functional requirement of the Mediator submodule Med7N/31.The EMBO journal · 2009Article
- Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription.Genes & development · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
TFIIS promotes the intrinsic ability of RNA polymerase II to cleave the 3'-end of the newly synthesized RNA. This stimulatory activity of TFIIS, which is dependent upon Rpb9, facilitates the resumption of transcription elongation when the polymerase stalls or arrests. While TFIIS has a pronounced effect on transcription elongation in vitro, the deletion of DST1 has no major effect on cell viability. In this work we used a genetic approach to increase our knowledge of the role of TFIIS in vivo. We showed that: (1) dst1 and rpb9 mutants have a synthetic growth defective phenotype when combined with fyv4, gim5, htz1, yal011w, ybr231c, soh1, vps71, and vps72 mutants that is exacerbated during germination or at high salt concentrations; (2) TFIIS and Rpb9 are essential when the cells are challenged with microtubule-destabilizing drugs; (3) among the SDO (synthetic with Dst one), SOH1 shows the strongest genetic interaction with DST1; (4) the presence of multiple copies of TAF14, SUA7, GAL11, RTS1, and TYS1 alleviate the growth phenotype of dst1 soh1 mutants; and (5) SRB5 and SIN4 genetically interact with DST1. We propose that TFIIS is required under stress conditions and that TFIIS is important for the transition between initiation and elongation in vivo.
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Registered trials
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