ArticleMolecular and cellular biology1992
Role of GCR2 in transcriptional activation of yeast glycolytic genes.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Cis- and trans-regulatory factors contributing to divergent activity of the TDH3 promoter in Saccharomyces yeast.Molecular biology and evolution · 2026Article
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- Genome-scale metabolic modelling identifies reactions mediated by SNP-SNP interactions associated with yeast sporulation.NPJ systems biology and applications · 2025Article
- Exploration and characterization of hypoxia-inducible endogenous promoters in Aspergillus niger.Applied microbiology and biotechnology · 2021Article
- Transcriptomic Changes Induced by Deletion of Transcriptional RegulatorFrontiers in bioengineering and biotechnology · 2021Article
- Metabolic Changes Induced by Deletion of Transcriptional RegulatorMicroorganisms · 2020Article
- Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays.Nucleic acids research · 2020Article
- Cross-stress resistance in Saccharomyces cerevisiae yeast--new insight into an old phenomenon.Cell stress & chaperones · 2016Review
- Contribution of network connectivity in determining the relationship between gene expression and metabolite concentration changes.PLoS computational biology · 2014Article
- Quantitative- and phospho-proteomic analysis of the yeast response to the tyrosine kinase inhibitor imatinib to pharmacoproteomics-guided drug line extension.Omics : a journal of integrative biology · 2012Article
- Inferring Transcriptional Interactions by the Optimal Integration of ChIP-chip and Knock-out Data.Bioinformatics and biology insights · 2009Article
- Transcriptional regulation of carbohydrate metabolism in the human pathogen Candida albicans.PLoS pathogens · 2009Article
- An improved map of conserved regulatory sites for Saccharomyces cerevisiae.BMC bioinformatics · 2006Article
- Regulation of glycolysis in Kluyveromyces lactis: role of KlGCR1 and KlGCR2 in glucose uptake and catabolism.Current genetics · 2004Article
- Trehalose, glycogen and ethanol metabolism in the gcr1 mutant of Saccharomyces cerevisiae.Folia microbiologica · 2003Article
- The Saccharomyces cerevisiae 14-3-3 protein Bmh2 is required for regulation of the phosphorylation status of Fin1, a novel intermediate filament protein.The Biochemical journal · 2002Article
- Rap1p requires Gcr1p and Gcr2p homodimers to activate ribosomal protein and glycolytic genes, respectively.Genetics · 2001Article
- The role of Gcr1p in the transcriptional activation of glycolytic genes in yeast Saccharomyces cerevisiae.Genetics · 1997Article
- Specialized Rap1p/Gcr1p transcriptional activation through Gcr1p DNA contacts requires Gcr2p, as does hyperphosphorylation of Gcr1p.Genetics · 1997Article
- Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae GCR2 gene affects expression of most of the glycolytic genes. We report the nucleotide sequence of GCR2, which can potentially encode a 58,061-Da protein. There is a small cluster of asparagines near the center and a C-terminal region that would be highly charged but overall neutral. Fairly homologous regions were found between Gcr2 and Gcr1 proteins. To test potential interactions, the genetic method of S. Fields and O. Song (Nature [London] 340:245-246, 1989), which uses protein fusions of candidate gene products with, respectively, the N-terminal DNA-binding domain of Gal4 and the C-terminal activation domain II, assessing restoration of Gal4 function, was used. In a delta gal4 delta gal80 strain, double transformation by plasmids containing, respectively, a Gal4 (transcription-activating region)/Gcr1 fusion and a Gal4 (DNA-binding domain)/Gcr2 fusion activated lacZ expression from an integrated GAL1/lacZ fusion, indicating reconstitution of functional Gal4 through the interaction of Gcr1 and Gcr2 proteins. The Gal4 (transcription-activating region)/Gcr1 fusion protein alone complemented the defects of both gcr1 and gcr2 strains. Furthermore, a Rap1/Gcr2 fusion protein partially complemented the defects of gcr1 strains. These results suggest that Gcr2 has transcriptional activation activity and that the GCR1 and GCR2 gene products function together.
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