ArticleJournal of virology2004
A novel motif in geminivirus replication proteins interacts with the plant retinoblastoma-related protein.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 100 citations in OpenAlex.
- Reexamining a Host-Associated Genomic Diversity of Bean Golden Mosaic Virus (BGMV) Isolates fromPathogens (Basel, Switzerland) · 2025Article
- Article
- Article
- REPercussions: how geminiviruses recruit host factors for replication.Frontiers in microbiology · 2023Review
- Metagenomic identification of novel viruses of maize and teosinte in North America.BMC genomics · 2022Article
- Characterization of Transcriptional Responses to Genomovirus Infection of the White Mold Fungus,Viruses · 2022Article
- The GpIA7 effector from the potato cyst nematode Globodera pallida targets potato EBP1 and interferes with the plant cell cycle.Journal of experimental botany · 2021Article
- Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications.Biology · 2021Review
- Article
- Roles of plant retinoblastoma protein: cell cycle and beyond.The EMBO journal · 2020Review
- Plant tumors: a hundred years of study.Planta · 2020Review
- Geminivirus Resistance: A Minireview.Frontiers in plant science · 2020Review
- Chilli leaf curl virus infection downregulates the expression of the genes encoding chloroplast proteins and stress-related proteins.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Infectivity of cloned begomoviral DNAs: an appraisal.Virusdisease · 2019Article
- Multifunctional roles of geminivirus encoded replication initiator protein.Virusdisease · 2019Article
- Identification of aViruses · 2018Article
- S-acylation of a geminivirus C4 protein is essential for regulating the CLAVATA pathway in symptom determination.Journal of experimental botany · 2018Article
- Improving agroinfiltration-based transient gene expression inPlant methods · 2018Article
- Fangorn Forest (F2): a machine learning approach to classify genes and genera in the family Geminiviridae.BMC bioinformatics · 2017Article
- The Involvement of Heat Shock Proteins in the Establishment ofFrontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The geminivirus replication factor AL1 interacts with the plant retinoblastoma-related protein (pRBR) to modulate host gene expression. The AL1 protein of tomato golden mosaic virus (TGMV) binds to pRBR through an 80-amino-acid region that contains two highly predicted alpha-helices designated 3 and 4. Earlier studies suggested that the helix 4 motif, whose amino acid sequence is strongly conserved across geminivirus replication proteins, plays a role in pRBR binding. We generated a series of alanine substitutions across helix 4 of TGMV AL1 and examined their impact on pRBR binding using yeast two-hybrid assays. These experiments showed that several helix 4 residues are essential for efficient pRBR binding, with a critical residue being a leucine at position 148 in the middle of the motif. Various amino acid substitutions at leucine-148 indicated that both structural and side chain components contribute to pRBR binding. The replication proteins of the geminiviruses tomato yellow leaf curl virus and cabbage leaf curl virus (CaLCuV) also bound to pRBR in yeast dihybrid assays. Mutation of the leucine residue in helix 4 of CaLCuV AL1 reduced binding. Together, these results suggest that helix 4 and the conserved leucine residue are part of a pRBR-binding interface in begomovirus replication proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.